Molecular Identification of Defoliatiny Pathotype and Non-defoliating Pathotype of Verticillium dahliae from Cotton in Hebei Province

doi:10.7668/hbnxb.2010.04.041

Abstract

Abstract: Two hundred and thirty three strains of V.dahliae and three strains of Fusarium oxysporum f. sp. vasinfectum were isolated from cotton of the major cotton production areas in Hebei，Sichuan and Liaoning provinces. The defoliating and nondefoliating pathotypes of these strains were evaluated by PCR amplification. Three pairs of primers， which are specific for identifying V.dahliae，defoliating and nondefoliating pathotypes，respectively，were used in the PCR analysis. Results showed that the V.dahliae-specific bands were amplified from all the 233 strains of V. dahliae isolates，but not the 3 strains of F.oxysporum f. sp. vasinfectum，by using V. dahliae specific primers DB19 /DB22. PCR amplification with nondefoliating pathotype-specific primers INTNDf / INTNDr revealed that strains Sch-1，Sch-3 from Sichuan province and strain Ly-1 from Liaoning province are nondefoliating type. Defoliating pathotype-specific DNA bands (450 bp) were amplified with primers INTD2f / INTD2r from all the 228 strains of V. dahliae from Hebei province， as well as the defoliating standard strain T9. Our results indicated that defoliating pathotype of V. dahliae was dominant in the cotton growing area of Hebei province.

Key words: Verticillium dahliae, Defoliating pathotype, Non-defoliating pathotype, Molecular identification

CLC Number:

S435.62

WANG Yan, LU Xiu-yun, GUO Qing-gang, LI Bao-qing, ZHANG Nai-jin, LI She-zeng, MA Ping, DONG Jin-gao. Molecular Identification of Defoliatiny Pathotype and Non-defoliating Pathotype of Verticillium dahliae from Cotton in Hebei Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 196-200. doi: 10.7668/hbnxb.2010.04.041.

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