Gene Structure Identification and Protein Sequence Bioinformation Analysis for GA2ox from Apical Tissue of Apple Shoots

doi:10.3969/j.issn.1000-7091.2012.05.011

Abstract

Abstract: GA 2-oxidase(GA2ox)is a critical enzyme in the pathway of gibberellins biosynthesis.In this research,GA2ox gene was isolated from Fuji stem apical tissue by homologous cloning technology.The cDNA sequence of GA2ox was 1 656 bp in length with 3 exons and 2 introns.Its open reading frame was 1 014 bp which encoding a protein of 337 amino acids.Molecular weight and isoelectric point of the protein was 37.679 8 kDa and 6.41,respectively.Conserved domains showed that the enzyme belonged to the class 2-oxoglutarate-dependent dioxygenases.Amino acids homology analysis indicated that the sequence had 51%-97% similarity with those of other reported plants.Cluster analysis showed that GA2ox from pear was clustered together with GA2ox from apple firstly,and followed by that from black cottonwood.

Key words: Apple(Malus domestica), GA2ox, Conserved domains, Amino acid homologous analysis, Cluster analysis

CLC Number:

S661.1

BAI Mu-dan, WANG Cai-hong, TIAN Yi-ke, CHEN Bao-yin, LIU Yun-long. Gene Structure Identification and Protein Sequence Bioinformation Analysis for GA2ox from Apical Tissue of Apple Shoots[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 55-59. doi: 10.3969/j.issn.1000-7091.2012.05.011.

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