Analysis of Screening Wheat Drought Resistance Varieties by IRAP

doi:10.7668/hbnxb.2014.03.020

Abstract

Abstract: Using IRAP molecular method, design primers and 36 wheat varieties were used for PCR amplification, polymorphic finger maps were obtained and clustering analysis.The results were as follows:246 bands were produced by IRAP, the range of amplification fragments were 250-2 000 bp, a total of 13 polymorphic loci, the range of different varieties was 0-12.the results of clustering analysis can be clearly differentiate drought resistance between 36 wheat varieties.The study showed that IRAP were suitable for screening drought resistance varieties in wheat.

Key words: IRAP molecular marker, Wheat, Drought resistance, Cluster analysis

CLC Number:

S512.03

LI Wei-tao, CHEN Qiu-fang, YA Hui-yuan, GU Yun-hong. Analysis of Screening Wheat Drought Resistance Varieties by IRAP[J]. journal1, 2014, 29(3): 105-108. doi: 10.7668/hbnxb.2014.03.020.

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http://www.hbnxb.net/EN/Y2014/V29/I3/105

References

[1] 郭凤芝,刘凤洲,郭凌云,等.小麦抗旱性品种筛选与分析[J].山东农业科学,2013,45(1):46-48.
[2] 魏添梅,昌小平,闵东红,等.小麦抗旱品种的遗传多样性分析及株高优异等位变异挖掘[J].作物学报,2010,36(6):895-904.
[3] 敬礼恒,刘利成,梅坤,等.水稻抗旱性能鉴定方法及评价指标研究进展[J].中国农学通报,2013,29(12):1-5.
[4] 杨琳,景继海,赵佰图.旱地小麦抗旱性鉴定指标研究[J]现代农业科技,2009(17):19-20.
[5] 赵会君,张怀刚,王海庆.抗旱性不同的春小麦品种籽粒萌发期α-淀粉酶活性及其同工酶分析[J].麦类作物学报,2008,28(4):633-637.
[6] 张文英,柳斌辉,彭海城,等.小麦品种抗旱性鉴定指标遗传规律研究[J].华北农学报,2008,23(增刊):92-95.
[7] 贾寿山,朱俊刚,王曙光,等.山西小麦地方品种萌发期的抗旱性[J].华北农学报,2011,26(2):213-217.
[8] 张立生,温辉芹,程天灵,等.小麦高产抗旱育种实践[J].山西农业科学,2011,39(12):1243-1246.
[9] Kalendar R, Grob T,Regina M,et al.IRAP and REMAP:two new retrotransposon-based DNA finger-printing techniques[J].Theor Appl Genet,1999,98:704-711.
[10] Kumar A,Bennetzen J I.Plant retrotransposons[J].Annu Rev Genet,1999,33:479-532.
[11] Leigh F,Kalendar R,Lea V,et al.Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques[J].Mol Genet Genom,2003,269(4):464-474.
[12] Petr Smykal.Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification[J].J Appl Genetic,2006,47(3):221-230.
[13] Breto M P,Ruiz C,Pina JA.The diversification of Citrus clementina Hort.ex Tan.,a vegetatively propagated crop species[J].Mol Phylogenet Evol,2001,21(2):285-293.
[14] Gao L,McCarthy E M,Ganko E W,et al.Evolutionary history of Oryza sativa LTR retrotransposons:a preliminary survey of the rice genome sequences [J].BMC Genomics,2004,5(1):18.
[15] 侯清松,车京玉,邵立刚,等.小麦抗旱性遗传与研究进展[J].小麦研究,2013,34(2):6-13.
[16] Kristin A,Schneider,Mary E B,et al.Marker assisted selection to improve drought resistance in common Bean[J].Crop Sci,1997,37:51-60.

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