A Study on Sex Determination of Bovine Pre-implantation Embryos by Multi-PCR

doi:10.7668/hbnxb.2011.06.017

Abstract

Abstract: Basing on the sequence of bovine Y-specific repeat sequence，5 pairs of bovine male-specific PCR primers were designed，meanwhile basing on the microsatellite genes，3 pairs of bovine-specific PCR primers were designed as internal control primers． Two pairs primers of Y-specific and 1 pairs of bovine-specific were selected basing on the single PCR amplification of bovine DNA，one pairs of primer combinations BY1 /A1 was established for sex identification by Multi-PCR amplification of bovine genomic DNA and embryos．

Key words: Bovine, Sex identification, Multi-PCR, Embryos

CLC Number:

S823． 03

WANG Xue-qing, PEI Cui-juan, LI Kui-ying, WANG Yin-qian, WANG Kun, ZHANG Feng, WU Zhan-jun. A Study on Sex Determination of Bovine Pre-implantation Embryos by Multi-PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 89-93. doi: 10.7668/hbnxb.2011.06.017.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.06.017

http://www.hbnxb.net/EN/Y2011/V26/I6/89

References

[1] Veerhuis R，Hendriksen P J，Hengst A M， et al. The production of anti-H-Y monoclonal antibodies: their potential use in a sex test for bovine embryos[J]. Vet Immunol Immunopathol， 1994， 42(3-4): 317-330.
[2] 陈从英，黄路生，陈静波，等. 牛早期胚胎性别鉴定 PCR 反应体系的优化研究[J]。畜牧兽医学报， 2003， 34(3): 209-212.
[3] Schroder A，Mi11er J，Thomsen P， et al. Sex determination of bovine embryos using the polymerase chain reaction [J]. Animal biotechnol， 1991，1: 121-133.
[4] 王宗礼，王栋，程金华等. 鉴别牛早期胚胎性别PCR方法引物的设计与筛选[J]. 畜牧兽医学报，2005，36(2): 1116-120.
[5] 任克良，梁全忠，毛杨毅. PCR 法鉴别牛胚性别技术研究[J]. 山西农业科学， 2000， 28(2): 3-6.
[6] 唐克礼. 家畜性别控制的方法和前景[J]. 山西农业科学， 1987(10): 38-39.
[7] 淮亚红，辛晓玲，昝林森，等. LAMP 法在牛早期胚胎性别鉴定中的应用[J]. 河南农业科学，2005(8): 91- 94.
[8] 魏红芳，秦粉菊，徐照学，等. 家畜性别控制与胚胎性别鉴定技术在畜牧业中的应用[J]. 河南农业科学， 2001(1): 27-28.
[9] 向前，刘玉婵. 性别控制在牛繁殖中的实践[J]. 河南农业科学， 1996(1): 30-31.
[10] J 萨姆布鲁克D W，拉塞尔. 分子克隆实验指南[M].第3 版. 北京: 科学出版社， 2002.
[11] Utsumi K，Hayashi M，Takakura R， et al. Embryo sex selection by a rat male specific antibody and the cytogenetic and developmental confirmation in cattle embryos[J]. Mol Reprod Dev， 1993， 34(1): 25-32.
[12] Hare W C D，Mitchell D，Betteridge K J， et al. Sexing 2- week old bovine embryos by chromosomal analysis prior to surgical transfer: preliminary methods and results[J]. Theriogenology， 1976，5 (5): 243-253.
[13] Hasler J F，Cardey E，Stokes J E，et al. Nonelect rophoretic PCR-sexing of bovine embryos in a commericial environment[J]. Theriogenology，2002，58(8): 1457-1469.
[14] 朱化彬. PCR 法鉴别哺乳动物胚胎性别的研究[J].国外畜牧科技， 1995， 22(5): 26-28.
[15] Park J H，Lee J H，Choi K M， et al. Rapid sexing of preimplantation bovine embryo using consecutive and multiplex polymerase chain reaction(PCR) with biopsied blastomere[J]. Theriogenology，2001，55(9): 1843- 1853.
[16] 黄银花，胡晓湘，徐慰倬，等. 影响多重PCR 扩增效果的因素[J]. 遗传， 2003， 25(1): 65-68.
[17] Bej A K，Mahbubani M H，Miller R， et al. Multiplex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water[J]. Mol Cell Probe， 1990，4 (5): 353-365.
[18] Sunzeri F J，Lee T H，Brownle R G， et al. Rapid simultaneous detection of multiple retroviral DNA sequence using the polymerase chain reaction and capillary DNA chromatography[J]. Blood， 1991， 77(4): 879-886.

Please choose a citation manager

Content to export