Abstract:
Polyclonal antibody(IgG) was isolated from antiserum against the recombinant coat proitein(CP) of PLRV by the treatment of saturated ammmonium sulfate， then crude IgG was purified with protein G affinity chromatography column． High purity IgG was achieved and labelled with alkaline phosphatase by one-step method of glutaraldehyde， a nd enzyme-conjugated IgG(IgG-AP) was obtained． When PLRV-infected potato leaf was detected by DAS-ELISA procedure using the prepared IgG and IgG-AP，positive reaction was observed，and there was no positive reaction in the detection of healthy leaf． The result indicated that the IgG against the recombinant CP and the resulting IgG-AP were successfully used in DAS-ELISA detection of potato leafroll virus．
Key words:
Potato leafroll virus(PLRV),
Recombinant CP,
Polycolonal antibody,
Phosphatase-conjugated IgG,
DAS-ELISA
CLC Number:
LI Nan-nan, ZUO Yu-ling, SUI Jiong-ming, GAI Shu-peng, FAN Lian-mei, LI Guang-cun, GUO Bao-tai. Application of Polyclonal Antibody against the Recombinant CP to the DAS-ELISA Detection of Potato Leafroll Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 85-88. doi: 10.7668/hbnxb.2011.06.016.