Cloning and Sequencing of gGGene of Duck Plague Virus HN Strain

doi:10.7668/hbnxb.2011.06.018

Abstract

Abstract: Duck Plague is an acute contagious and highly lethal virus disease of ducks． According to published Duck Plague Virus gGgene sequences，one pair of specific primers was designed and synthesized． The genomic DNA of DPV Henan strain，which was isolated from Henan，was used as template． Full-length gGgene was amplified by polymerase chain reaction(PCR) and cloned into the PMD19-T vector． The gene was sequenced and the open reading frame of 1 380 bp was determined． it encode 459 amino acids and the predicted protein has an molecular mass of 50． 5 kDa. The clone of gGgene provides foundation for study of its biological function．

Key words: Duck Plague Virus(DPV), gGgene, Cloning, Sequencing

CLC Number:

S858． 31

WANG Yan, CAO Su-fang, CUI Bao-an. Cloning and Sequencing of gGGene of Duck Plague Virus HN Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 94-96. doi: 10.7668/hbnxb.2011.06.018.

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http://www.hbnxb.net/EN/Y2011/V26/I6/94

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