Establishment of ISSR-PCR Reaction System in Chinese Jujube

doi:10.7668/hbnxb.2008.S2.048

Abstract

Abstract: Take the genomic DNA of Ziziphus jujuba Mill. cv. Dongzao as a template, the ISSR??PCR reaction system of Chinese jujube was estabblished after analysised the effect of 6 main factors in PCR react ion using the single factor method.The opt imal ISSR??PCR reaction system was determined as follows: 1 PCR Buffer, 2.0 mmol/L Mg²⁺, 0.25mmol/ L dNTP, 15 U Taq polymerase, 125 ??mol/ L primer and 50 ng template DNA in total 25 L reaction volume. The opimal annealing temperature was 58℃. All the result established a foundation for building cultivar fingerprinting and genetic linkage map in Chinese jujube by using ISSR molecular markers.

Key words: Chinese jujube, ISSR, System optimization

CLC Number:

S665.1

QI Jing, DONG Zhen, SHEN Lian-ying, MAO Yong-min, CHEN Liang. Establishment of ISSR-PCR Reaction System in Chinese Jujube[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 209-212. doi: 10.7668/hbnxb.2008.S2.048.

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