ACTA AGRICULTURAE BOREALI-SINICA ›› 2008, Vol. 23 ›› Issue (S2): 213-216. doi: 10.7668/hbnxb.2008.S2.049

Special Issue: Grape

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Computational Identification of Novel microRNAs in Grape

CAI Bin1, LI Cheng-hui2, PENG Ri-he3, XIONG Ai-sheng3, GAO Feng3, YAO Quan-hong3, ZHANG Zhen4   

  1. 1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
    2. Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, China;
    3. Biotechnology Research Institute of Shanghai Academy of Agriculture Sciences, Shanghai 201106, China
  • Received:2008-09-05 Published:2008-12-31

Abstract: microRNAs ( miRNAs) represent a new family of non-protein-coding small RNAs ( 20- 25nt ) that functionby degrading targeted mRNAs or repressing mRNA translation. Here we developed a program-MirFinder for predictionof grape miRNAs.The characteristic features of known plant miRNAs were used as criteria to search for miRNAs. Aftersearching the grape genome, MirFinder ident ified 146 miRNAs, of which 98 miRNAs were the same as known miRNAsand 48 are new ident ified miRNAs.The 48 miRNAs were classified into 21 families, of which 8 families are newly identified in grape. A total of 15 potential targets were identified for 6 of the 8 new miRNA families based on the fact that miRNAs exhibit perfect or nearly perfect complementarity with their target sequences

Key words: Grape genome, microRNA, Bioinformat ics

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Cite this article

CAI Bin, LI Cheng-hui, PENG Ri-he, XIONG Ai-sheng, GAO Feng, YAO Quan-hong, ZHANG Zhen. Computational Identification of Novel microRNAs in Grape[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 213-216. doi: 10.7668/hbnxb.2008.S2.049.

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