Genetic Diversity of ISSR Markers and Environment Factors Correlation Analysis of Medicago ruthenica in China

doi:10.7668/hbnxb.2014.06.017

Abstract

Abstract: In order to provide a theoretical basis and technical support for the protection and utilization of M.ruthenica,genetic diversity and environment factors correlation analysis were studied using ISSR molecular markers of 49 M.ruthenica collected from 7 provinces or autonomous regions of China.The results showed that 15 primers were selected which had clear polymorphic bands.A total of 127 bands,including 110 polymorphic bands,were detected by using the 15 selected primers,and the proportion of polymorphic fragment was 85.61%,averaged 7.33 bands for each primer pair.Nei's gene diversity index,Shanaon diversity and Gst of within pupolutions were greater than those of within regions.The genetic diversity was rich in Inner Mongolia and Tibet,the genetic differences in the Beijing was smaller.Based on cluster and principal component analyses,49 populations could be divided into 6 groups. M.ruthenica germplasm resources presented a good regional distribution.Genetic diversity index,Shannon index and the percentage of polymorphic loci were significantly negatively correlated with annual rainfall.

Key words: M.ruthenica, ISSR, Genetic diversity, Environment factors, Correlation

CLC Number:

S551.03

LI Zhi-yong, LI Hong-yan, CAI Li-yan, SHI Wen-gui, HUANG Fan, LIU Lei, XIE Yong-feng. Genetic Diversity of ISSR Markers and Environment Factors Correlation Analysis of Medicago ruthenica in China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 94-100. doi: 10.7668/hbnxb.2014.06.017.

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http://www.hbnxb.net/EN/Y2014/V29/I6/94

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