Appraisal of Medicago Ruthenica Germplasms by SSR Markers

doi:10.7668/hbnxb.2008.03.018

Abstract

Abstract: Eighteen reproducible and informative SSR primer pairs were selected from the 89 pairs of primers of Medicago truncatula.In 8 genome DNA of Medicago ruthenica.And a representative set of 50 wild Medicago ruthenica genetic resources from Inner Mongolian were analyzed by SSR methodology using the informative primer paris for their diversity of genetic.The results showed a total of 109 alleles were detected with an average of 6.1 alleles per SSR primer pair.According to integrated evaluation,6 SSR primer pair,BI4BO3,MTIC272,MAL369471,MTIC237,MTIC188 and MTIC27 were most effective for genetic diversity studies on Medicago ruthenica.The genetic distances among the germplasms ranged between 0.023 6 and 0.807 5 with their average at 0.177 8.By cluster analysis with sum of squares for microsatellite showed that 50 accessions can be divided into 9 groups with different characteristics which could afford reference in deeper research on Medicago ruthenica.

Key words: Medicago ruthenica, M icrosatellite, Polymorphism, Cluster anlysis

CLC Number:

LI Hong-yan, LI Zhi-yong, MI Fu-gui, LU Xin-xiong, SHI Wen-gui. Appraisal of Medicago Ruthenica Germplasms by SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 67-71. doi: 10.7668/hbnxb.2008.03.018.

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