Molecular Cloning and Expression Analysis of Delta 1-pyrroline-5-carboxylate Synthetase(P5CS) Gene in Puccinellia chinampoensis

doi:10.7668/hbnxb.2011.06.005

Abstract

Abstract: Delta 1-pyrroline-5-carboxylate synthetase(P5CS) plays a critical role in improving the stress tolerance of plants． In this study， the full length cDNA sequence of P5CS gene was cloned from Alkaligrass(Puccinellia chinampoensis) leaves using RT-PCR method， the primers were designed according to the homologous P5CS gene sequences of other plant species． Sequence analysis showed that the nucleotide sequence of this gene is 2 158 bp，containing a complete open reading frame and encoding 716 amino acids，Genebank: HQ637435． Nucleotide and amino acid sequence analysis revealed that PuP5CSshares high identity with the orthologs from Triticum aestivum． And its signal peptide，hydrophobicity /hydrophilic， trans-membrane domain，secondary structure and main functional domains were predicted． Semi-quantitative RT-PCR analysis showed that PuP5CSexpressed in different tissues，but the expression in root was lower，and in leaf much higher． Various elevated levels of PuP5CSexpression have been detected when exposed to 4 different stress experimental treatment，and the results was not the same．

Key words: Puccinellia chinampoensis, P5CS, Gene clone

CLC Number:

XU Bo, REN Wei, XU An-kai, WANG Zhi-feng, SUN Qi-zhong. Molecular Cloning and Expression Analysis of Delta 1-pyrroline-5-carboxylate Synthetase(P5CS) Gene in Puccinellia chinampoensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 20-26. doi: 10.7668/hbnxb.2011.06.005.

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