Studies of Watermelon Genetic Diversity Using SRAP and EST-SSR

doi:10.7668/hbnxb.2010.03.018

Abstract

Abstract: The genetic diversities were studied using 30 SRAP primer pairs in 9 cultivated watermelon lines,3 American lines,6 African wild lines.The results implied that the polymorphic percentage between cultivated lines and wild lines was 92.11%, the polymorphic percentage in wild lines was 34.11%, and in cultivated lines was 21.05%.The bands of American lines were mostly the same as cultivated lines＇and some specific bands were amplied.The results showed that SRAP and EST-SSR were highly effective and feasible on watermelon studying．

Key words: Wild watermelon, Cultivated watermelon, Polymorphic, SRAP, EST-SSR

CLC Number:

S651.03

ZHAO Sheng-jie, LIU Wen-ge, YAN Zhi-hong, HE Nan, BAO Wen-feng. Studies of Watermelon Genetic Diversity Using SRAP and EST-SSR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 76-79. doi: 10.7668/hbnxb.2010.03.018.

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References

[1] Zhang X P,Rhodes B B.RAPD molecular marker in watermelon[J].Hort Science,1993,28(5):223.
[2] Lee S L,Shin J S,Park K,et al.Detection of genetic diversity using RAPD-PCR and sugar analysis in watermelon(C.lanatus)germplasm[J].Theor Appl Genet,1996,92:719-725.
[3] Levi A,Thomas C E,Wehner T C,et al.Low genetic diversity indicates the need to broaden the genetic base of cultivated watermelon[J].Hort Science,2001,36(6):1096-1101.
[4] Levi A,Thomas C E,Keinath A P,et,al.Genetic diversity among watermelon (Citrullus lanatus and Citrullus colocynthis) accession[J].Genet Resources Crop Evol,2001,48:559-566.
[5] 张安世,邢智峰,韦慧彦,等.苔藓植物SRAP反应体系的优化[J].河南农业科学,2009(11):108-111.
[6] 郭大龙,侯小改,张静,等.牡丹SRAP反应体系的建立及正交设计优化[J].河南农业科学,2008(12):110-113.
[7] 李晓慧,王从彦,徐小利,等.西瓜SRAP-PCR反应程序的建立与体系的优化[J].华北农学报,2008,23(3):38-41.
[8] Ferriol M,Pico B,Nuez F.Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers[J].Theor Appl Genet,2003,107:271-282.
[9] Li G,Quiros C F.Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theor Appld Genet,2001,103:455-461.
[10] 张军,武耀廷,郭旺珍,等.棉花微卫星标记的PAGE/银染快速检测[J].棉花学报,2000,12(5):267-269.
[11] 李晓慧,王从彦,张四普,等.西瓜二倍体及同源多倍体SRAP多态性分析[J].分子植物育种,2007,5(6):61-63.
[12] 李严,张春庆.西瓜杂交种遗传多态性的SRAP标记分析[J].园艺学报,2005,32(4):643-647.
[13] Levi A,Thomas C E,Trebitsh T,et al.An extended linkage map for watermelon based on SRAP,AFLP,SSR,ISSR and RAPD markers.[J].Amer Soc Hort Sci,2006,131(3):393-402.

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