Study on Bovine Somatic Cell Nuclear Transfer

doi:10.7668/hbnxb.2008.05.012

Abstract

Abstract: The research was designed to explore a brief procedure on nuclear transfer,which included compare of di-f ferent methods for mature culture of bovine occytes,separation and general culture of fibroblast taken from the skin of bovine,enucleated method and condit ion on fusion which exactly affect the development of reconstructed embryos.Finally the highest mature rate was 81.7% with FSH and LH added,and fusion rate was 93.96% with voltage of 2.6 kV/cm.The cleavage rate and 8-cell development rate of embryos the percentage was 61.2% and 3.27% respect ively when two-half cytoplas-t method was applied,and the data up to 70.8% and 7.56% while blind-cutting manipulation was adopted,and these outcome were all obtained under the condition that reconstructed embryos were cultured in pressing WOW after activation.

Key words: Somatic cell, Nuclear transfer, Handmade cloning, Bovine

CLC Number:

S823

JIA Rui-zhen, LI Yu, YANG Li-jun, HUANG Xiang-hua. Study on Bovine Somatic Cell Nuclear Transfer[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 53-57. doi: 10.7668/hbnxb.2008.05.012.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.05.012

http://www.hbnxb.net/EN/Y2008/V23/I5/53

References

[1] Wilmut I,Schmieke A E,McWhir J,et al.Viable offspring derived from fetal and adult mammalian cells[J].Nature,1997,385:810-813.
[2] Vogel G.Cloned gaur a short-lived success[J].Science,2001,291:409.
[3] 安晓荣,苟克勉,关宏,等.卵丘细胞核移植技术生产克隆牛犊[J].中国科学:C辑,2002,32(1):69-76.
[4] 谭世俭.5头经玻璃化冷冻保存后移植的成年体细胞克隆牛降生[J].广西农业生物学,2003,22(4):334.
[5] 龚国春,戴蕴平,朱化彬,等.供体细胞类型对体细胞克隆牛生产效率的影响[J].中国科学:C辑,2004,34(3):257-262.
[6] Bronson R A,McLaren A.Transfer to the mouse oviduct of eggs with and without the zona pellucida[J].Reprod Fertil,1970,22(1):129-137.
[7] Modlinski J A.The role of the zona pellucida in the development of mouse eggs in vivo[J].Embryol Exp Morphol,1970,23(3):539-47.
[8] Trounson A O,Moore N W.The survival and development of sheep eggs following complete or partial removal of the zona pellucida[J].Reprod Fertil,1974,41(1):97-105.
[9] Peura T T,Lewis I M,Trounson A O.The effect of recipient oocyte volume on nuclear transfer in cattle[J].Mol Reprod Dev,1998,50(2):185-91.
[10] Vajta G,Lewis I M,Hyttel P,et al.Somatic cell cloning without micromanipulators[J].Cloning,2001,3(2):89-95.
[11] Peura T T.Improved in vitro development rates of sheep somatic nuclear transfer embryos by using a reverse-order zona-free cloning method[J].Cloning Stem Cells,2003,5(1):13-24.
[12] Thouas G A,Jonesand G M,Trounson A O.The 'GO' system-a novel method of microculture for in vitro development of mouse zygotes to the blastocyst stage[J].Reproduction,2003,126(2):161-169.
[13] Lagutina I,Lazzari G,Duchi R,et al.Somatic Cell Nuclear transfer in horses:effect of oocyte morphology,embryo reconstruction method and donor cell type[J].Reproduction,2005,130:559-567.
[14] Du Y,Kragh P M,Zhang Y,et al.Piglets born from handmade cloning,an innovative cloning method without micromanipulation[J].Theriogenology,2007,68:1104-1110.

[1]	ZHANG Kang, ZHANG Kai, WANG Lei, CUI Dongan, ZHANG Jingyan, YAN Zunxiang, MA Xueqing, XUE Huan, LI Jianxi. *Isolation and Identification of Bovine viral diarrhea virus* Strain GSLY** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 378-384.
[2]	MA Peng, LI Linjie, CHANG Qiuyan, WANG Yueying, GUO Fucheng, LIU Ping, MA Xiaoxia, MA Zhongren. The Developments of SYBR Green Ⅰ Real-time PCR and Conventional RT-PCR for Detection of BVDV [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 49-53.
[3]	LI Yan-qing, NIU Xiao-liang, HU Jiang, YAN Wei, LUO Yu-zhu, WANG Wen-biao, CAIRANG Nao-ri. *Polymorphism of BoLA-DRA^Exon2-intron2 in Yak and Bovine** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 72-77.
[4]	KAN Wei, WANG Hua, MA You-ji, ZHAO Xing-xu, ZHANG Yong. Construction of Eukaryotic Expression Vector of cfb Gene from Bovine Streptococcus agalactiaee [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 39-44.
[5]	HUANG Cheng-jun, GUO Lin-lin, SUN Shou-qiang, LIN Feng, LIU Hai-jun. *Effect of Cumulus Cells on in vitro* Maturation of Bovine Oocytes** [J]. journal1, 2014, 29(4): 111-115.
[6]	CHEN Jing-ting, BU Deng-pan, MA Lu, YANG Jin-hui, LI Fa-di. Comparative Analysis of Immunoglobulin and Lactoferrin in Different Bovine Milk [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 298-302.
[7]	SUN Wei, LI Xi-he, GUO Ji-tong, BA Te-er, YANG Li-guo. Influence of Epidermal Growth Factor on Cell Proliferation Cycle and Apoptosis of Cultured Bovine Oviductal Epithelial Cells in vitro [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 410-414.
[8]	WANG Li-qin, WANG Jing, ZHAO Yun-cheng, LIN Jia-peng, HUANG Jun-cheng. Effect of Donor Cells and Processing on the Efficiency of Sheep Nuclear Transfer [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 69-73.
[9]	WANG Guo-fu, WU Hui-guang, SUN Guo-quan, LI Jun-ya, GAO Xue, WANG Jing-shan, WANG Yu-quan, GAO Shu-xin. Association Analysis of Polymorphism in the Bovine Lactoferrin Gene Exon11 with the Susceptibility to Mastitis and Part Milk Trait in Simmental Cattles [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 218-222.
[10]	LI Fen, ZHOU Pei, YANG Ting, CHEN Ning-bo, MA Yun. *Bioinformatics Analysis of Bovine ANGPTL2* Gene Structure and Function** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 51-56.
[11]	WANG Xue-qing, PEI Cui-juan, LI Kui-ying, WANG Yin-qian, WANG Kun, ZHANG Feng, WU Zhan-jun. A Study on Sex Determination of Bovine Pre-implantation Embryos by Multi-PCR [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 89-93.
[12]	LIU Tao, LI Xiang-chen, GUAN Wei-jun, MA Yue-hui. The Influence of Bovine Oocytes in Vitro Maturation and Apoptosis by CdCl_2 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 146-152.
[13]	WANG Lan-ping, GENG Rong-qing, JI De-jun, CHANG Hong, CHANG Chun-fang, LI Yong-hong. Analysis of Genetic Co}daptability in Variation Sites of Bovine Myostatin Gene [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 51-53.
[14]	LI Xi-yan, WANG Jia-qi, WEI Hong-yang, BU Deng-pan, HU Han, ZHOU Ling-yun. BovineMammary EpithelialCells CultureMethods in vitro and Its Application [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 31-35.
[15]	LI Xi-yan, WANG Jia-qi, WEI Hong-yang, BU Deng-pan, HU Han, ZHOU Ling-yun. The Optimal Culture Conditions in vitro for Bovine Mammary Epithelial Cells [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 79-85.

Please choose a citation manager

Content to export