Abstract: Folliclestimulating hormone beta(FSHB)subunit gene and estrogen receptor alpha(ESRA)subunit gene were selected as two candidates to screen the genomic variation by means of PCR2SSCP and sequences mensuration in blue eggshell chicken(85 individuals),and the association of FSHBand ESRAgenes polymorphisms with early egg production traits were analyzed.Polymorphisms were detected in FSHB and ESRA genes,the results showed that three nucleot ide variation at A2464G,2450A insertion,A2405G,were identified in FSHBpromotor region,and a nucleot ide varia2 tion at A2447G in FSHBexon 3;Four SNPs,A234G,T276C,T279C,C2.02T,were identified in ESRA5c flanking regulation region,and three nucleot ide variation at T.22C,A.55G,C.69T in ESRA intron..Association analysis of early egg production traits showed that the egg number at 36 weeks of genotype AA was higher than that of AB(p<0.05),genotype BB was not found in FSHB promotor region;Moreover,in exon 3 site,the age at first egg was also significant difference between AA and AB(p<0.05),neither genotype BB was found.The egg number at 36 weeks was significant difference between CC and CD genotypes of ESRA5cflanking regulation region(p<0.05)in blue eggshell chicken,genotype DD was not found.Rest is no difference(p>0.05).

Key words: Chicken, FSHB subunit gene, ESRAsubunit gene, Egg product ion traits

CLC Number: 

• S831.2