Effect of Donor Cells and Processing on the Efficiency of Sheep Nuclear Transfer

doi:10.3969/j.issn.1000-7091.2012.z1.014

Abstract

Abstract: Study of lamb fibroblast， fetal fibroblast， embryonic stem cells and treatment methods on the efficiency of nuclear transplantation effect． Compared the lamb skin fibroblasts hunger and balance time on the cell availability effect; Compared the fetal fibroblast starvation and balance time on the cell availability effect; Compared thelamb fibroblasts， fetal fibroblast， embryonic stem cells in the availability and the fusion rate; Compared the embryonic stem cells and transgenic lambs skin fibroblast on the efficiency of nuclear transfer． The result indicated starvationon the availability rate of fetal fibroblast have little effect(P ＞ 0． 05) ; But have great impact on the balance time(P ＜0． 01 ) ; The effect of hunger and balance time on fetal fibroblasts were very significant ( P ＜ 0． 01 ) ; Fetal fibroblastcell availability was significantly higher than those of embryonic stem cells and lamb skin fibroblasts ( P ＜ 0． 01 ) ，The fusion rate is significantly higher than that of the lamb ( P ＜ 0． 05) ， but have no difference on embryonic stemcells ( P ＞ 0． 05) ． After embryo transfer， embryonic stem cells in pregnancy rates were significantly higher than thatof sheep skin fibroblasts ( P ＜ 0． 05) ． Hunger and balancing time for donor cells have significant impact on availability． The effect of embryonic stem cell nuclear transfer is better than fibroblasts．

Key words: Nuclear transfer, Fibroblast cell, Embryonic stem cell, Starvation, Balance time

CLC Number:

Q813

WANG Li-qin, WANG Jing, ZHAO Yun-cheng, LIN Jia-peng, HUANG Jun-cheng. Effect of Donor Cells and Processing on the Efficiency of Sheep Nuclear Transfer[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.014.

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