Studies on Embryo Cloning in Boer Goat

doi:10.7668/hbnxb.2007.04.017

Abstract

Abstract: The aim of the present study was to carry out nuclear transfer with the Boer goat embryo blastomeres as nuclear donors, to compare the effects of the sources of recipient oocytes and development stages of donor embryos on the fusion rate of reconstructed embryo; to investigate if Vero cell monolayers improved the development of reconstructed embryo in vitro; the reconstructed embryos developed to term. The fusion rate of reconstructed embryos from in vivo or in vitro matured oocytes was 85.7% and 88.7% respectively, there was no significant difference between them (P>0.05). The fusion rate of reconstructed embryos with blastomeres from 8, 16, 32 cell embryos was 91.6%, 86%, 89.2%, there was no significant difference among them (P>0.05). Co-cultured with Vero cell monolayers, the reconstructed embryos obtained higher clevaged and morula rate (65.5%, 32.7%) than that of simply cultured in TCM199 (53.1%, 10.6%)(P<0.05). Eighteen fused reconstructed embryos were transferred into 2 recipients, no pregnancy was confirmed; three morula cultured in vivo were transferred into 1 recipient, the recipient was confirmed pregnant (33%), the pregnance was maintained through term, and gave birth a female kid. The kid weighed 3.45 kg, and grew to 42.5 kg one years old. The cloned goat gave birth a twin Boer kids by natural mating, this is the first report of cloned Boer goat produced by embryo cell nuclear transfer.

Key words: Goat, Embronic cells, Nuclear transfer, Electric fusion, Cloning, Boer goat

CLC Number:

S827.9+2

LIU Hai-jun, LIU Ling, FENG Jian-zhong, LIU Yu-tang, XUE Jun, ZHANG Jin-long, ZHANG Xiao-sheng, LI Ping, CHI Bing-jie. Studies on Embryo Cloning in Boer Goat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 71-75. doi: 10.7668/hbnxb.2007.04.017.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.04.017

http://www.hbnxb.net/EN/Y2007/V22/I4/71

References

[1] Willadsen S M. Nuclear transplantation in sheep embryo[J]. Nature, 1986, 227: 298-300.
[2] 张涌, 王建辰, 钱菊汾, 等. 山羊卵核移植的研究[J]. 中国农业科学, 1991, 24(5): 1-6.
[3] 邹贤刚, 李光三, 王玉阁, 等. 山羊(Capra hircus)胚胎细胞经继代细胞核移植后其发育能力的研究[J]. 科学通报, 1995, 40(3): 264-267.
[4] Zhang Y, Li Y Q. Nuclear-cytoplasmic interaction and development of goat embryosreconstructed by nuclear transplantation: production of goats by serially cloning embryos[J]. Biol Reprod, 1998, 58: 266-269.
[5] Prather R S, Barnes F L, Simes M L. Nuclear transfer in the bovine embryo: assessment of donor nuclei and recipient oocytes[J]. Biol Reorod, 1987, 37: 859-866.
[6] Prather R S, Sims M M, First N L. Nuclear transplantation in early pig embryos[J]. Biol Reorod, 1989, 41: 414-418.
[7] Meng L, Ely J J, Stouffer R L, et al. Rhesus monkeys produced by nuclear transfer[J]. Biology of Reproduction, 1997, 57: 454-459.
[8] Reggio B C, James A N, Green H L, et al. Cloned transgenic offspring resulting from somatic cell nuclear transfer in the goat: oocytes derived from both follicle-stimulating hormonestimulated and nonstimulated abattoir-derived ovaries[J]. Biology of Reproduction, 2001, 65: 1528-1533.
[9] Bondioli K R, Westhusin M E, Looney C R. Production of identical bovine offspring by nuclear transfer[J]. Theriogenology, 1990, 33: 165-174.
[10] Collas P, Robl J M. Development of rabbit nuclear transplant embryos from morula and blastocyst stage donor nuclei[J]. Theriogenology, 1991, 35 (1): 190.
[11] 刘凤军, 安志兴, 高立功, 等. 优化山羊体细胞核移植方案的研究[J]. 畜牧兽医学报, 2006, 37(10): 972-976.
[12] Menezo Y, Dumont M, Hazout A, et al. Regulation and limitation of in vitro blastocyst formation in human. In implantation in mammals. (L Gianaroli, A Campana, AO Trounson. eds)[M]. New York, USA: Raven Press, Serono Symposia publications, 1992: 167-176.
[13] Menck M C, Guyader-Joly C. Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems[J]. Reprod Nutr Dev, 1997, 37: 141-150.
[14] Zou X G, Chen Y, Wang Y G, et al. Production of cloned goats from enucleated oocytes injected with cumulus cell nuclei or fused with cumulus cells[J]. Cloning, 2001, 3 (1): 31-37.
[15] Zou X G, Wang Y G, Cheng Y, et al. Generation of cloned goats(Capra hircus) from transfected foetal fibroblast cells, the effect of donor cell cycle[J]. Molecular Reproduction and Development, 2002, 61: 164-172.

Please choose a citation manager

Content to export