Study on the Expression ofBMPRIBmRNA Mongolian Cattle Ovary

doi:10.7668/hbnxb.2008.05.013

Abstract

Abstract: Specific primers was designed by conserver sequence of the other animals,total RNA was extracted from ovary of cow and the cDNA encoding cowBMPRIBwas amplified by the reverse transcription PCR(RT-PCR),then the purified RT-PCR product was cloned into PMD18-T vector.The results of PCR analysis of recombinant plasmid and DNA sequencing demonstrated that the cowBMPRIBwas belong to the family of bone morphogenetic protein,BMPRIBmRNA was specialized expressed in cow fore primary and secondary follicle and follicle granulose cell,and expressed terminal secondary follicle by using situ hybridization.

Key words: Mongolian cow, BMPRIB, RT-PCR, In situ hybridization

CLC Number:

S823

WANG Feng, LIU Yong-bin, RONG Wei-heng, TIAN Chun-ying, DA Lai. Study on the Expression ofBMPRIBmRNA Mongolian Cattle Ovary[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 58-61. doi: 10.7668/hbnxb.2008.05.013.

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http://www.hbnxb.net/EN/Y2008/V23/I5/58

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