Abstract: Wheat Fusarium head blight(FHB),caused mainly by Fusarium graminearum and Fusarium culmorum,is a serious disease problem.The resistance resoure to FHB,however,is very poor.USDA geneticist Steven Xu produced a set of lines [LDN(PI481521)].Each line had a different pair of chromosome from PI481521 substituted for the corresponding Langdon durum chromosomes.The purpose of this study was to determine if any of the LDN(PI481521) lines showed useful level of resistance of FHB.We test these lines for response by inoculating with Fusarium graminearum in the greenhouse.Inoculation was accomplished via the single spikelet method.In the test,some lines differed significantly.Two lines,LDN(PI481521-3A) and LDN(PI481521-7B),were consistently less susceptible and another two, LDN(PI481521-2A) and LDN(PI481521-6A),were more susceptible than the parent Langdon durum,which itself showed an intermediate FHB reaction.Also,these inoculated spikelets were detected by using Beacon Analytical Systems DON plate kit to analysis the mycotoxin.The results showed two lines LDN(PI481521-3A) and LDN(PI481521-7B) were the lowest amount of mycotoxin among the substitution lines,So there is a strong correlation between FHB severity and Don contents.Consequently,the genes responsible for or resistant to FHB and inhibition of mycotoxin production was preliminary localization.

Key words: Wheat, Wheat substitution lines, Fusarium gramdrcearum, Resistance identification, Mycotoxin

CLC Number: 

• S12.01