SSR Analysis of Powdery Mildew Resistance G ene in New Wheat G ermplasm N95175

doi:10.7668/hbnxb.2008.01.044

Abstract

Abstract: To definitude genetic effects and gene loci of powdery mildew resistance gene in new wheat2haynalidiavillosa germplasm N95175,conventional analyze and SSR technology was used to analyze heredity and molecular markersof resistant gene. The results showed that F1of N95175 × Shaan160 and N95175 × Shaanyou225 were highly resistant topowdery mildew. The ratios of resistance plants to susceptible plants in F2generation were 115∶ 43 and 111∶ 48,respec2tively. The segregation ratio fitted expected 3∶ 1,so it suggested that powdery mildew resistance of N95175 was controlledby a dominant gene.By SSR analysis with 208 pairs of primers,two markers Xgwm570 and Xwmc553 generated polymor2phic DNA fragments between the resistant and susceptible plants DNA pools. By analyzing the polymorphic markers inthese segregating populations,Xgwm570 and Xwmc553 were linked to the resistance gene with genetic distance of 13138and 12103 cM. The genetic distance between marker Xgwm570 and Xwmc553 was calculated as 3174 cM.By using twomarkers,the marker certification was analyzed in N95175 × Shaan160 F2generation,the according rate was 89124 % com2pared with inoculation. According to chromosome loci of Xgwm570 and Xwmc553,powdery mildew resistance gene inN95175 was located on wheat 6A chromosome.

Key words: New wheat germplasm, Powdery mildew resistance, Genetic effects and genetic mapping, SSR marker

CLC Number:

S435.12

MA Ying-ying, WANG Chang-you, JI Wan-quan, WANG Qiu-ying, LIU Xue-li. SSR Analysis of Powdery Mildew Resistance G ene in New Wheat G ermplasm N95175[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 199-203. doi: 10.7668/hbnxb.2008.01.044.

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