Preliminary Research on Pathogenic Secretions Generated by Ditylenchus destructor Thorne

doi:10.7668/hbnxb.2008.01.045

Abstract

Abstract: To explore Ditylenchus destructor Thorne pathogenic mechanism, a preliminary study was conducted withprotein secretions of D. destructor. After nematode glands growth was stimulated by 5 -methoxy -N, N -dimethyltryptamineand dimethyl sulfoxide, nemade was grinded on ice and secretory granules were acquired. The proteins from the granuleswere abstracted by TCA/Acetone and ammonium sulfate precipitation method. Then pathogenicity of protein was testedwith sweet potato slices.The results showed that the secretions from nematode esophageal gland, especially proteins obv- iously played a pathogenic role. Crude proteins abstracted by ammonium sulfate precipitation had the stronger pathogenicitythan that from TCA/acetone method. And the pathogenic crude proteins were heat sensitive and ultraviolet radiation sens- itive, they almost completely lost pathogenicity at 100? for 30min withwater bath and under 40w, 50 cm UV light expo -sure for 60 min.

Key words: Ditylenchus destructor, Pathogenicity, Secretion, Protein

CLC Number:

S435.313+.4

GONG Wei-bo, JIANG Ji-zhi, WANG Xing-zhe. Preliminary Research on Pathogenic Secretions Generated by Ditylenchus destructor Thorne[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 204-206. doi: 10.7668/hbnxb.2008.01.045.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.01.045

http://www.hbnxb.net/EN/Y2008/V23/I1/204

[1]	JIN Yifeng, GAO Yansong, WANG Qi, WANG Mengmeng, ZHAO Di, XIONG Yi, CHEN Yang. *Cloning of SnRK2.4* Gene and It's Response to Abiotic Stress in Kentucky Bluegrass** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 9-15.
[2]	HAN Xiaoyu, LI Qinglun, JIANG Xinglin, WANG He, YANG Lingling, SHI Yajuan, LI Honglian, CHEN Linlin, YANG Xue, SHI Yan. Localization,Interaction and Pathogenicity of BYDV GAV Encoded Proteins [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 150-156.
[3]	SU Fang, HAN Diangang, YE Lingling, ZHANG Chong, YIN Shanglian, LUO Qianmin, DONG Xianlan, LI Yaoyao, LI Lingfeng, AI Jun, XIN Jige. Expression of COVID-19 Virus N Gene in Insect Cells [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 174-180.
[4]	DENG Jie, WANG Hanlin, LI Youyu, WANG Zie, CHEN Xiaohua, LIU Diqiu. *Cloning and Analysis of a Polygalacturonase Inhibiting Protein Gene and Its Promoter from Lilium regale* Wilson** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 45-52.
[5]	GUO Fangrui, LIU Yi, YANG Kexin, CHU Meirong, ZHANG Quanfa, ZHANG Yafei, WANG Shutong, CAO Keqiang. *Screening of Fusarium oxysporum* T-DNA Inserted Mutants and Analysis of Inserting Genes** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 206-212.
[6]	ZHU Yiming, LIU Yanxiao, HE Jiuqing, DUAN Lingtao, ZHOU Erxun. Studies on Functions of Effector Gene ChEP085 in Colletotrichum higginsianum [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 192-200.
[7]	JIA Weizhe, JIAO Bo, WANG Jiao, CHEN Wenye, YANG Fan, LIU Yongwei, DONG Fushuang, ZHAO Liqun, ZHOU Shuo. *Cloning and Expression Analysis of Calmodulin-like Protein TaCML8-A* Gene in Wheat** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 1-8.
[8]	ZHANG Jian, WANG Na, LIU Zhida, BAO Tingting, ZHANG Zhiwei, YUN Xiaopeng, SHI Bixian, ZHANG Heng, CHEN Guihong, CHEN Yanfang, ZHAO Jun. Identification and Pathogenicity Differentiation of the Pathogens Causing Necrotic Spot on Sunflower Broomrape [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 172-180.
[9]	CHEN Wenxun, ZHOU Ting, YAN Qiongxian, TIAN Lina, CAO Manhu, TAN Zhiliang, ZOU Aihua. Effect of Low Protein Diet on Meat Quality and Myogenic Regulatory Factor Gene Expression of Xiangdong Black Goats [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 202-210.
[10]	WANG Xianjun, XIANG Hua, ZHANG Xumei, REN Yupeng, ZHU Jiangjiang. Effects of ORFV118 on Cell Cycle,Apoptosis and the Immune-related Cytokines in Goat Testicular Sertoli Cells [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 222-231.
[11]	SONG Xiangjun, SONG Zichao, SHEN Xiao, CHEN Zhe, JIANG Huyan, HOU Manman, SHAO Ying, TU Jian, QI Kezong. *Effects of Avian Pathogenic Escherichia coli* Hcp2b on the Expression Profile of Spleen mRNA in Chicken Spleen** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 232-238.
[12]	FENG Lei, WEI Weiqun, ZHONG Xueqing, FU Mingjia, XIAO Shiping, YE Dexiao, HUANG Ziyan. *Prokaryotic Tandem Expression and Antibody Preparation of Subtilomycin Structural Gene subA* from Bacillus subtilis SX3411** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 347-352.
[13]	CHEN Mei, CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHONG Jincheng, XIN Jinwei. *Cloning,Protein Function and Tissue Expression Analysis of Yak CTGF* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 361-367.
[14]	GAO Qian, FENG Yan, YANG Yahua, ZHAO Qingsong, LEI Yakun, LIU Bingqiang, ZHANG Mengchen, SHI Xiaolei, YANG Chunyan. Detecting QTL Underlying Wild Soybean Protein Content Through Genome Wide Association Study [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 23-30.
[15]	LONG Yanxi, LUO Yanru, ZHU Zhenghang, LIAO Zhiyi, WANG Lan. Research on Protein Content and Glutelin Gene Expression in Tetroploid Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 78-87.

Please choose a citation manager

Content to export