Segregation Distortion and Genetic Diversity in Homoeologous Group 1 of Wheat RIL Population

doi:10.3969/j.issn.1000-7091.2012.04.013

Abstract

Abstract: To know better the genetic diversity in homoeologous group 1 of wheat, a recombinant inbred line （RIL） population derived from a cross between two winter wheat cultivars, Longjian 19 and Q9086, was used to study the segregation distortion and the genetic diversity in homoeologous group 1 of wheat by SSR marker. The re- suits showed that twenty three pairs of SSR markers selected in 97 pairs of SSR primers were polymorphic between two parents. The polymorphic frequency in different chromosomes of homoeologous group 1 presented 1B （30.30%）〉 1A （28.57 % ）〉 1D （ 10.34% ）. Total of 107 polymorphic markers were identified in RIL population with a range from 2 to 10 and a mean of 4.65 per each SSR primer. Polymorphism information content（PIC） ranged from 0. 326 to 0. 880 with 1B（0. 709）〉 1A（0. 534）〉 1D（0. 498）. Genetic similarity coefficient（GS） ranged from 0.40 to 0.96. According to SSR polymorphic markers, the RIL could be divided into nine subgroups. The ratio of parental genotypes was segregated into 1：1.13 in RIL population. Total of 15 SSR markers showed the significantly segrega- tion distortion（P 〈 0.05） with 65.2% of segregation frequency by Chi-square test,favoring either the marker al- leles of male parent Longjian 19 （7 SSR markers） or female parent Q9086 （8 SSR markers）. Among these segrega- ted SSR markers, six segregated markers was distributed on chromosome 1A,seven markers on 1 B and two on 1D.

Key words: Wheat, RIL population, SSR Genetic diversity, Segregation distortion

CLC Number:

S512.3

AN Mei, YANG De-long, LI Meng-fei, ZHANG Guo-hong, LI Wei. Segregation Distortion and Genetic Diversity in Homoeologous Group 1 of Wheat RIL Population[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 73-79. doi: 10.3969/j.issn.1000-7091.2012.04.013.

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References

[1] Zhang L,Sun G,Yan Z,et al. Comparison of newly syn- thetic hexaploid wheat with its donors on SSR products [J]. Journal of Genetics and Genomics, 2007,34 ( 10 ) : 939 -946.

[2] Somers D J, Isaac P, Edwards K. A high-density wheat mi- crosatellite consensus map for bread wheat( Triticum aesti- vum L. ) [J]. Theoretical and Applied Genetics, 2004, 109:1105 - 1114.

[3] Quarrie S A, Steed A, Calestani C, et al. A high-density genetic map of hexaploid wheat ( Triticum aestivum L. ) from the cross Chinese Spring × SQ1 and its use to com- pare QTLs for grain yield across a range of environments [J]. Theoretical and Applied Genetics, 2005,110 : 865 - 880.

[4] Su J, Zheng Q, Li H, et al. Detection of QTLs for phos- phorus use efficiency in relation to agronomic perform- ance of wheat grown under phosphorus sufficient and limited conditions [J]. Plant Science, 2009,176 : 824 - 836.

[5] Wu X,Wang Z, Chang X,et al. Genetic dissection of the developmental behaviours of plant height in wheat under diverse water regimes[J]. Journal of Experimental Bota- ny,2010,61(11) :2923 -2937.

[6] Yang D, Jing R, Chang X, et al. Identification of quanti- tative trait loci and environmental interactions for accu- mulation and remobilization of water-soluble carbohy- drates in wheat( Triticum aestivum L. ) stems[J]. Genet- ics,2007,176:571 - 584.

[7] Yang D, Jing R, Chang X, et al. Quantitative trait loci mapping for chlorophyll fluorescence and associated traits in wheat ( Triticum aestivum L. ) [J]. Journal of Integrative Plant Biology,2007,49 ( 5 ) :646 - 654.AA29

[8] Li H,Tong Y, Li B,et al. Genetic analysis of tolerance to photo-oxidative stress induced by high light in winter wheat( Triticum aestivum L. ) [J]. Journal of Genetics and Genomics,2010,37:399 - 412.

[9] Peng J H, Fahima T, R~der M S,et al. Microsatellite high density mapping of the stripe rust resistance gene YrH52 region on chromsome 1B and evaluation of its marker as- sisted selection in the F2 generation in wild emmer wheat [J]. New Phytologist,2000,146 ( 1 ) : 141 - 154.

[10] Wang L F,Ma J X,Zhou R H,et al. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat [J]. Euphytica,2002,124 ( 1 ) : 71 - 73.

[11] Smith J S C,Chin E C L, Shu H,et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L. ) :comparisons with data from RFLPs and Pedigree[J]. Theoretical and Applied Genetics, 1997, 95:163 - 173.

[12] Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants:Present status and future prospects[J]. Plant Molecular Biology,2005, 57:461 -485.

[13] Liu Z H, Anderson J A, Hu J,et al. A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utihty for detecting quantitative trait loci [J]. Theoretical and Ap- plied Genetics,2005,111:782 - 794.
[14] Roder M S,Korzun V,Wendehake K,et al. A microsatel- lite map of wheat[J]. Genetics, 1998,149:2007 - 2023.

[15] Lu H, Romero-Severson J, Bernardo R. Chromosomal re- gions associated with segregation distortion in maize [J]. Theoretical and Applied Genetics,2002,105:622 -628.

[16] Xu Y,Zhu L, Xiao J,et al. Chromosomal regions associ- ated with segregation distortion of molecular markers in F2,backcross, doubled haploid, and recombinant inbred populations in rice( Oryza sativa L. ) [J]. Molecular Ge- netics and Genomies, 1997,253:535 - 545.

[17] Matsushita S, Iseki T, Fukuta Y,et al. Characterization of segregation distortion on chromosome 3 induced in wide hybridization between indica and japonica type rice vari- eties [J]. Euphytica,2003,134:27 - 32.

[18] Konishi T, Yano Y, Abe K. Geographic distribution of al- leles at the Ga2 locus for segregation distortion in barley [J]. Theoretical and Applied Genetics, 1992,85:419 - 422.

[19] Blanco A,Bellomo M P,Cenci A,et al. A genetic linkage map of durum wheat[J]. Theoretical and Applied Genet- ics, 1998,97:721 - 728.

[20] Messmer M M, Keller M, Zanetti S,et al. Genetic linkage map of a wheat spelt cross[J]. Theoretical and Applied Genetics, 1999,98 : 1163 - 1170.

[21] Paillard S,Schnurbusch T, Winzeler M, et al. Anintegra- tire genetic linkage map of winter wheat ( Triticum aesti- vum L. ) [J]. Theoretical and Applied Genetics, 2003, 107 : 1235 - 1242.

[22] 《普通小麦B基因组的研究进展》[J].分子植物育种,2008(4).

[23] 《小麦RIL群体SSR分子标记偏分离的遗传分析》[J].麦类作物学报,2011(3).

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