Studies of the Methods for Total RNA Isolation from Plantlets of Apple Cultured in vitro

doi:10.3321/j.issn:1000-7091.2006.z1.012

Abstract

Abstract: The CTAB-guanidinium method, modified CTAB method, and RNAplant Kit method were used to extract the total RNA from the plantlets of apple cultured in vitro, in which contain a lot of carbohydrates and phenolic compound. The CTAB-guanidinium method showed the best results in term of the quality and quantity of yielded RNA with the A₂₆₀/A₂₃₀ value of more than 2.0, A₂₆₀/A₂₈₀ ranged from 1.8 to 2.0, and the total RNA yield of 16.95μg/g. The modified CTAB and RNAplant Kit methods were a bit short in total RNA extraction because of a little DNA contamination. And the RNAplant Kit method showed advantages of fastness and convenience.

Key words: Apple, Total RNA, Isolation, in vitro

CLC Number:

S661.1

ZHANG Xin, DU Guo-qiang, SHI Xiao-xin. Studies of the Methods for Total RNA Isolation from Plantlets of Apple Cultured in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 50-53. doi: 10.3321/j.issn:1000-7091.2006.z1.012.

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