Construction of Genetic Modified Pseudomonas fluorescens Which Habouring aiiA Gene from Bacillus thuringiensis and Its Activity Against Soft Rot Disease

doi:10.3321/j.issn:1000-7091.2006.04.004

Abstract

Abstract: aiiA protein,encoded by aiiA gene,can inactivates the AHL signals which regulates in quorum sensing system in bacteria by hydrolyzing its lactone bond so that it can release the disease in plant.Engineered strain P303-ss10 which can control bacterial and fungi diseases was constructed in this paper by transforming aiiA gene into Pseudomonas fluorescens P303.DNA fragments in 0.8 kb size were obtained by PCR and RT-PCR and it indicated that the engineered strain P303-ss10 was harbouring aiiA gene.P303-ss10 not only remained strong antifungal activity against several kinds of plant pathogenic fungi as P303,but also could control soft rot diseases in potato and Chinese cabbage effectively in vivo.

Key words: Engineered Pseudomonas fluorescens, Quroum sensing, aiiA gene, Erwinia.carotovora pv.carotovora, Bacterial disease control

CLC Number:

ZHANG Xia, HU Yu-qin, JIA Zhen-hua, ZHANG Jie, MA Hong, SONG Shui-shan. Construction of Genetic Modified Pseudomonas fluorescens Which Habouring aiiA Gene from Bacillus thuringiensis and Its Activity Against Soft Rot Disease[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 13-16. doi: 10.3321/j.issn:1000-7091.2006.04.004.

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