Abstract:
In order to get donor cells for somatic cells nuclear transfer,The experiment researched that is separate skin flbroblast of Yorkshire and out -of -body grow characteristic of origin and subculturing.The growth curve of culture cell in vitro appeared “S” shape.The cell survival rate descended but most of them recovered after cryopreservation,We gained cell lines that can normally serial subcultive in vitro which offer cell resources for animal clone research.
Key words:
Yorkshire,
Fibroblastcellline,
Cryopreservation,
Biologicalcharacteristics
CLC Number:
MAHong. Establishment and Biological Characteristics of a Yorkshire Piglets Skin Fibroblast Cell Line[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.024.