Study on Preparation of Monoclonal Antibodies Against Kanamycin and Development of Indirect Competitive Inhibition ELISA

doi:10.7668/hbnxb.2010.02.023

Abstract

Abstract: The antigen of kanamycin synthesized by EDC reaction was used for preparation of a hybridoma cell line.which secretes monoclonal antibodies against kanamycin.The titers of antibody in cell supematant and ascites were 1∶103 and 1∶5×104,respectively.A competive ELISA test was developed with anti-KAN monoclonal antibody and the sensibivity of 0.5 ng/mL,the IC50 of 3.7 ng/mL.The antibody against kanamycin was selective toward tobramycin with cross-reactivity of about 63.8%,and,wasn't detective cross-reactivity toward others.The ELISA rapidity,sensitivity,specificity and briefness and was proved to be used in the rapid test of KAN residues in animal food.

Key words: Kanamycin, Monoclonal antibody, EL ISA

CLC Number:

Q813.2

QI Hong-li, YANG Guang, LIU Jin-lan. Study on Preparation of Monoclonal Antibodies Against Kanamycin and Development of Indirect Competitive Inhibition ELISA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 121-124. doi: 10.7668/hbnxb.2010.02.023.

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http://www.hbnxb.net/EN/Y2010/V25/I2/121

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