Development and Traits Identification of Colloidal Gold-Labeled Monoclonal Antibody Rapid Detection Strip of Streptomycin

doi:10.7668/hbnxb.2010.05.018

Abstract

Abstract: For the development of colloidal gold-labeled monoclonal antibody rapid detection strip of streptomycin,the monoclonal antibody against streptomycin was generated and the rapid test strip of streptomycin(STR-Strip) was developed by gold immunochromatography assay(GICA) . Its traits were tested. The results showed that the detection limit of STR-Strip was 12. 5 μg /kg by eyes,it had no cross-reactivity towards other antibacterials,its sensitivity was as same as that of competitive ELISA-Kit and its coincidence rate was 100% compared with ELISA-Kit. With the strip,the test results were showed in less than 10 min,the strip possessed sensitivity,specificity and briefness. The strip was proved to be used for the rapid test of STR residues in animal food.

Key words: Streptomycin, Gold-labeled rapid detection strip, Monoclonal antibody, Traits identification

CLC Number:

LIU Qingtang, ZHI Aimin, DENG Ruiguang, YANG Yanyan, YANG Jifei, CHAI Shujun, ZHANG Gaiping. Development and Traits Identification of Colloidal Gold-Labeled Monoclonal Antibody Rapid Detection Strip of Streptomycin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 85-89. doi: 10.7668/hbnxb.2010.05.018.

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