Development of Rapid Test Blocking ELISA Kit for Sulfamethoxazole Residues

doi:10.7668/hbnxb.2009.02.044

Abstract

Abstract: A ELISA kit for detection of Sulfamethoxazole (SMZ-Kit)was developed with generating hybridoma lines that secrete SMZ monoclonal antibodies (SMZmAb) and the method of blocking ELISA,its traits were identified. The calibration curve of SMZ-Kit was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 1.0 to 128.0μg/ L (R² = 0.9901),the sensitivity of 0.73μg/ L,the IC₅₀ of 11.5μg/ L and the detection limit of 1.0μg/L.The recoveries of SMZ spiked in feed were 83.9 % and in milk were 83.3 %.The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation were below 15 %.The SMZ - Kit had 2.88 % cross-reactivity towards Sulfadiazine (SD) and little or no cross-reactivity towards other Sulfonamides.The validity of SMZ-Kit in 4℃was above six months.

Key words: Sulfamethoxazole, Monoclonal antibody, Blocking ELISA, Rapid test kit

CLC Number:

S859.8

LIU Xuan-bing, ZHANG Gai-ping, WANGBin, TENGMan, DENG Rui-guang, HOU Yu-ze, XING Guang-xu, YANGJi-fei. Development of Rapid Test Blocking ELISA Kit for Sulfamethoxazole Residues[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 214-217. doi: 10.7668/hbnxb.2009.02.044.

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