RNAi Plasmid Construction of Delta-12-fatty Acid Desaturase Gene from Peanut

doi:10.3321/j.issn:1000-7091.2006.04.003

Abstract

Abstract: An RNAi plasmid is constructed to inhibit the expression of delta-12-fatty desaturase gene in peanut,in order to down regulate the biosynthesis of linoleic acid and obtain transgenic lines with high oleic acid /linoleic acid ratio.The primers were designed based on the sequence of delta-12-fatty desaturase gene released in GenBank(AF248739),and the promoter region and partial extron of the gene in peanut was cloned using the genomic DNA from peanut cultivar Yuhua 4 as template.An RNAi plasmid pCAMBIA1301-Afad12Ri was constructed which contains the cloned promoter and inverted repeats of delta-12-fatty desaturase gene fragment.

Key words: Peanut (Arachis hypogaes), Delta-12-fatty desaturase gene, RNAi, hpRNA, Plasmid construction

CLC Number:

S565.2

CHEN Zhan-kuan, ZHANG Xin-you, MIAO Li-juan, HUANG Bing-yan, TANG Feng-shou, ZHANG Zhong-xin. RNAi Plasmid Construction of Delta-12-fatty Acid Desaturase Gene from Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 9-12. doi: 10.3321/j.issn:1000-7091.2006.04.003.

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