DistRibution ChaRicteRistics of Xylanase InhibitoR PRotein Activity in DiffeRent CeReal KeRnels

doi:10.3969/j.issn.1000-7091.2013.01.026

Abstract

Abstract: To obtain the accuRate infoRmation about the activity of xylanase inhibitoR pRotein in diffeRent ceReals and fuRtheR investigate its potential negative effect on the function of exogenous xylanase used in ceReal pRocessing， the keRnels of 9 commonly planted ceReals including wheat，baRley， Rye，gReen wheat，buckwheat，oats，coRn， Rice and soRghum weRe selected as expeRimental mateRials to study the xylanase inhibitoR pRotein activity and the activity distRibution in diffeRent fRactions． The Results showed that the xylanase inhibitoR pRotein activity in wheat， Rye，gReenwheat， a nd buckwheat( 4． 27，4． 76 4． 18 and 3． 61 IA/mg) was much higheR than in baRley and oats( 0． 54 and 0. 87 IA/mg) ． The xylanase inhibitoR pRotein activity weRe not detected in coRn， Rice and soRghum． Then wheat， Rye and gReen wheat with higheR xylanase inhibitoR activity weRe fuRtheR used to deteRmine the distRibution of xylanase inhibitoR pRotein activity in diffeRent fRactions of keRnel． The inhibiting activity in seed coat，embRyo and endospeRm weRe 0． 06，3 ． 67 and 4． 47 IA/mg in wheat Respectively，1 ． 70，5 ． 42 and 5． 39 IA/mg in Rye Respectively，and 0， 5. 06 and 4． 57 IA/mg in gReen wheat Respectively． It was concluded that the xylanase inhibitoR pRotein activity was much higheR in the embRyo and endospeRm than in the seed coat．

Key words: CeReal, KeRnels, Xylanase inhibitoR pRotein, Activity, DistRibution

CLC Number:

Q814

ZHANG Jun-li, ZHANG Ya-kun, LIU Xin-yu, WANG Ming-dao, LIU Liang-wei, CHEN Hong-ge. DistRibution ChaRicteRistics of Xylanase InhibitoR PRotein Activity in DiffeRent CeReal KeRnels[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 140-143. doi: 10.3969/j.issn.1000-7091.2013.01.026.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2013.01.026

http://www.hbnxb.net/EN/Y2013/V28/I1/140

References

1] Simpson D J，FincheR G B，Huang A H C， et al． StRuctuRe and function of ceReal and Related higheR plant( 1，4 ) -beta- xylan endohydRolases[J]． J CeReal Sci， 2003， 37: 111 - 127．

[2] DebyseR W，Peumans W J，Van Damme E J M， et al． TRiticum aestivum xylanase inhibitoR ( TAXI) ，a new class of enzyme inhibitoR affecting bReadmaking peRfoRmance[J]． J CeReal Sci， 1999， 30: 39 - 43．
[3] McLauchlan W R，GaRcia-Conesa M T，Williamson G， et al． A novel class of pRotein fRom wheat which inhibits xylanase
[J]． Biochem J， 1999， 338: 441 - 446．

[4] FieRens E，Rombouts S，GebRueRs K， et al． TLXI，a novel type of xylanase inhibitoR fRom wheat( TRiticum aestivum) belonging to the thaumatin family[J]． Biochem J，2007， 403( 3) : 583 - 591．

[5] Beliёn T，Van Campenhout S，Robben J， et al． MicRobial endoxylanases: Effective weapons to bReach the plant cellwall baRRieR oR， RatheR， tRiggeRs of plant defense systems
[J]． MoleculaR Plant-MicRobe InteRactions，2006，19 ( 10) : 1072 - 1081．

[6] 王明道，魏照辉，张俊丽，等小麦不同生育期木聚糖酶活性及木聚糖酶抑制活性的变化[J]麦类作物学报， 2010， 30( 3) : 544 - 547．

[7] PeRez-VendRell A M，BRufau J． Role of xylanase-inhibitoRs on nutRitive value of wheat-based diets supplemented with diffeRent xylanases foR poultRy[C]/ /14th EuRopean Symposium on PoultRy NutRition． NoRway: LillehammeR， 2003．

[8] Wang M，HameR R J， van Vliet T， et al． Effect of wateR unextRactable solids on gluten foRmation and pRopeRties: mechanistic consideRations[J]． J CeReal Sci， 2003， 37: 55 - 64．

[9] Wang M，HameR R J， van Vliet T， et al． InteRaction of wateR extRactable pentosans with gluten pRoteins: effect on dough pRopeRties and gluten quality[J]． J CeReal Sci， 2002， 36: 25 - 37 ．

[10] GoesaeRt H，Elliott G，Paul A， et al． OccuRRence of pRoteinaceous endoxylanase inhibitoRs in ceReals[J]． Biochim Biophys Acta， 2004， 1696: 193 - 202．

[11] GoesaeRt H． OccuRRence，puRification and pRopeRties of endoxylanase inhibitoRs in diffeRent ceReals[D]． Leuven: Katholieke UniveRsiteit Leuven， 2002．

[12] GebRueRs K，CouRtin C M，GoesaeRt H，et al． Endoxylanase inhibition activity in diffeRent EuRopean wheat vaRieties and milling fRactions theRe of[J]． CeReal Chem， 2002， 79: 613 - 616．

[13] Igawa T，Ochiai-Fukuda T，Takahashi-Ando N， et al． New TAXI-type xylanase inhibitoR genes aRe inducible by pathogens and wounding in hexaploid wheat[J]． Plant Cell Phys， 2004， 45: 1347 - 1360．

[14] CRoes E，GebRueRs K，Luyten N． Immunoblot quantification of thRee classes of pRoteinaceous xylanase inhibitoRs in diffeRent wheat( TRiticum aestivum) cultivaRs and milling fRactions[J]． J AgRi Food Chem，2009，57: 1029 - 1035．

[1]	LI Haodong, QIN Mengfan, ZHANG Miao, WANG Rongrong, XU Yu, SONG Jia, ZHU Yunlin, HUANG Zhen, XU Aixia. *Effects of Leaf Physiology and Pistil/Stamen Development of Brassica napus* L.to Low Temperature During the Flowering Stage** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 78-86.
[2]	LI Xia, LUO Lihui, ZHOU Ya, YANG Dingqing, WANG Peng, LI Sen. Effects of Straw Returning on Soil Active Organic Carbon Components and Enzyme Activities in Rice-Rape Rotation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 124-131.
[3]	ZHANG Zhiwei, FAN Junchen, KANG Liru, JIA Ruifang, TIAN Zaimin, ZHAO Jun. *Effect of StRab5b* Gene on Anthocyanin Synthesis in Potato** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 28-33.
[4]	DENG Jie, WANG Hanlin, LI Youyu, WANG Zie, CHEN Xiaohua, LIU Diqiu. *Cloning and Analysis of a Polygalacturonase Inhibiting Protein Gene and Its Promoter from Lilium regale* Wilson** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 45-52.
[5]	ZHANG Panpan, SHAO Yunhui, LIU Jingbao, QIAO Jiangfang, LI Chuan, ZHANG Meiwei, ZHAO Xia, HUANG Lu. Effect of Nitrogen and Zinc Application on the Accumulation and Distribution of Dry Matter and Nitrogen and Zinc of Different Summer Maize Varieties [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 96-103.
[6]	LI Jun, YAN Zhihao, JIA Wanli, XU Mengmeng, ZHANG Jingfeng, XU Qiuliang, HAN Haoyuan, QUAN Kai. *Identification and Transcriptional Activity Analysis of ATGL* Gene Promoter in Dairy Goat** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 223-230.
[7]	LI Fujian, XU Dongyi, LIU Kaili, ZHU Min, LI Chunyan, ZHU Xinkai, DING Jinfeng, GUO Wenshan. Effects of Tillage Methods on Growth and Physiology of Seedlings Stem and Tillers and Their Productivity of Wheat in Rice Stubble [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 58-67.
[8]	PING Huaixiang, CUI Jianyu, CHEN Shuo, WEI Lulu, CHEN Qing, ZHANG Delong. Effects of Fertilization on the Stoichiometry of Carbon,Nitrogen and Phosphorus and Enzyme Activities in Agricultural System [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 112-120.
[9]	YAN Hongmei, DUAN Qiuyu, LI Hongqiao, GUO Shixing, WU Yongcheng. Effect of Density on Dry Matter and Nitrogen Accumulation and Distribution of Dwarf Rape [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 143-148.
[10]	LIU Changyuan, JIAO Fengli, HONG Shengzhe, SUN Chitao, ZHANG Mingming, ZHANG Kai, LI Quanqi. Effects of Different Mulch Treatments on Soil Enzyme Activities and Soil Nutrients [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 246-252.
[11]	LI Ju, GAO Chengfei, MA Ning, WANG Shuya, LUO Shilei, Lü Jian, FENG Zhi, HU Linli, XIAO Xuemei, YU Jihua. Effects of Chemical Fertilizer Reduction Combined with Biological Organic Fertilizer on Nutrient Absorption and Yield of Cauliflower [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 153-162.
[12]	LI Jingyi, WANG Ren, MENG Xiangjie, LIANG Yugang, GONG Xiangsheng, HUANG Huang, WEI Hailin. Effects of No Tillage Semi-solid Direct Seeding on Physiological Characteristics of Flag Leaves of Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 87-98.
[13]	LUO Xiaoyu, SHAN Chenyang, JIA Zhaozhao, ZHANG Xin, LIN Ling. The Antifungal and Plant Growth-Promoting Effect and Components Analysis of the Volatile Organic Compounds from an Endophytic Bacterium S258 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 198-203.
[14]	PANG Na, CHENG Song, ZHANG Shuimei, YUAN Jingchao, LIU Jianzhao, LIU Songtao, REN Jun, LIANG Yao, CAI Hongguang. Effects of Chemical Fertilizer Combined with Organic Amendments on Soil Fertility and Spring Maize Yield in Mollisol [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 124-131.
[15]	QIAN Yuyuan, LIU Yi, ZHANG Xi, WANG Yan, WANG Guangen, CUI Shufang, LI Junlan. Transcriptome Analysis of Cotton Seed Weight Mutant ims-15 and Its Near Isogenic Line During Seed Development [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 50-59.

Please choose a citation manager

Content to export