Abstract: In the in vitro culture of the shoot tips from adult tree of two kinds of the Red Fujiyama apple, Changfu No. 2 and the Short- branch Fujiyama, the optimum temperature and light intensity for multiplication and rooting are different.As compared with other tested temperatures, 29℃ day temp, and 20℃ night temp. are more favorable for the adventitious bud differentiation and growth. The optimum rooting temperature is 25℃ day temp. and 18℃ night temp, but the optimum temperature for differentiation and growth of shoots is lower than themWhen the light intensity is 1500-2500 lux and the photoperiod is 10 hrsof light, the differentiatiation and growth of the shoots are the the best. Although a continuous illumination of 1500 lux is beneficial to differentiation and growth of the shoots. but the internodes are too short, the stem become purple and the effective shoots can ' t be formed.Light inhibits the rooting of shoots.The inhibitory degree is proportional to lightintensity from 600-2500 lux. Although the rooting rate is highest(90%) in darkculture the shoots are thin and weak. So the dark culture induced rooting is order to strengthen shoots and improve the survial rate in transplantation.The rooting rate with continuous illumination is higher than with discontinuous illumination of the same light intensity, but the roots are short and thin, root tips and stem b ecome purple, internodes are short. The shoots can recover to normal after 10 day if they are transferred to discontinuous light culture.

Key words: Apple, shoot tip in vitro culture, Temperature, Light intensity