Analysis of AGBL5 Expression in Testis and Epididymis of Bactrian Camels During the Breeding and Non-breeding Seasons

doi:10.7668/hbnxb.20196717

Abstract

Abstract:

To investigate the expression and distribution patterns of AGBL5 in the testis and epididymis tissues of Bactrian camels,as well as its regulatory role in seasonal reproduction,this study focused on AGBL5,a differentially expressed gene identified through transcriptomic screening,using tissues from the testis and epididymis of Bactrian camels during the breeding season and non-breeding season,using H&E staining,immunofluorescence(IF),Real-time Quantitative PCR(qRT-PCR)and Western Blot to examine the expression of the AGBL5 gene in testicular and epididymal tissues at different developmental stages.Sequencing results indicated that the AGBL5 gene was highly expressed at mRNA level in testicular tissue during the non-breeding season.H&E results showed that the lumen of the seminiferous tubules in testicular tissue was larger during the breeding season than during the non-breeding season,with a large number of spermatozoa present.The walls of the epididymal ducts were thicker during the breeding season,whereas the opposite was true during the non-breeding season.Spermatozoa were observed during both the breeding and non-breeding seasons;in epididymal tissue,the thickness of the epididymal duct walls increased during the breeding season,and the surrounding connective tissue appeared loose;during the non-breeding season,the epididymal duct walls were thinner and the connective tissue was denser,whilst a large accumulation of spermatozoa was visible within the lumen of the epididymal tail.qRT-PCR results showed that in testicular tissue,the mRNA expression level of the AGBL5 gene was significantly higher during the non-breeding season than during the breeding season.In epididymal tissue,the mRNA expression levels of the AGBL5 gene in both the head and tail of the epididymis were significantly higher during the non-breeding season than during the breeding season,while the mRNA expression levels in the body of the epididymis were significantly lower during the non-breeding season than during the breeding season.Western Blot results showed that AGBL5 protein expression levels were consistent with the trends observed for mRNA expression.IF results showed that the AGBL5 protein exhibited varying degrees of fluorescent expression signals in testicular interstitial cells,sertoli cells,epididymal epithelial cells and smooth muscle cells.In summary,the results suggest that the differential expression of AGBL5 may be closely related to testicular development and sperm maturation,laying a data foundation for further investigation into the role of AGBL5 in the regulation of seasonal reproduction in male Bactrian camels.

Key words: Bactrian camel, AGBL5, Differential expression, Testis, Epididymis

CLC Number:

MA Wenrui, NAN Jinghong, GE Tingting, LIU Yuanyuan, ZHANG Yong, ZHAO Xingxu, WANG Qi. Analysis of AGBL5 Expression in Testis and Epididymis of Bactrian Camels During the Breeding and Non-breeding Seasons[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(5): 230-238. doi: 10.7668/hbnxb.20196717.

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Figures/Tables 7

Tab.1 Primer sequence information

基因 Gene	引物序列(5'—3') Primer sequence (5'—3')	片段长度/bp Product size	扩增效率/% Amplification efficiency	退火温度/℃ Annealing temperature
GAPDH	F: AACATCATCCCTGCTTCTACC	184	93	58
	R: ATGCCTGCTTCACTACCTTCT
AGBL5	F: AACCCCAGGCAGGTCTACTT	173	108	59
	R: CTCTCTCCTGGGTGAACTCG

Fig.1 Analysis of transcriptional expression levels of the AGBL5 gene FQ.The estrus phase;NC.The anestrus phase.Different uppercase letters indicate extremely significant differences(P<0.01).The same as Fig.3,6.

Fig.2 H&E staining LC.Interstitial cells;ST.Spermatogenic tubules;PC.Peritubular myoid cells;PS.Primary spermatocytes;S.Spermatogonia;CV.Capillaries;CA.Cilia;BC.Basal cells;EEC.Epididymal epithelial cells;SZ.Spermatozoa;SMC.Smooth muscle cells.

Fig.3 Expression analysis of AGBL5 in Bactrian camel testes Different lowercase letters indicate significant differences(P<0.05).The same as Fig.4—6.

Fig.4 Expression analysis of AGBL5 in the head of the epididymis of Bactrian camel

Fig.5 Expression analysis of AGBL5 in the body of the epididymis of Bactrian camels

Fig.6 Expression analysis of AGBL5 in the tail of the epididymis of Bactrian camels

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