Gene Mapping for Leaf Rust Resistance in the Segregating Population of Common Wheat KU3067 and Apav

doi:10.7668/hbnxb.20196231

Abstract

Abstract:

Discovery and mapping of disease-resistant genes in wheat varieties can provide resistance sources,genes and markers for the breeding of new wheat cultivars.KU3067/Apav BC₁F₅ population were used to evaluate for leaf rust resistance at seedling and adult plant stage in Baoding,Hebei Province,and leaf rust resistance gene(s)were mapped using molecular marker technology.At the seedling stage,KU3067 was highly susceptible to three leaf rust physiological races while Apav was highly resistant.The 148 BC₁F₅ lines showed a separation ratio of one pair of genes,indicating that there was a dominant gene in Apav.It was confirmed that the gene was the known leaf rust resistance gene Lr13 using molecular marker technology.Both parents showed high resistance at the adult stage,and the population showed continuous distribution,which was consistent with the characteristics of quantitative traits.Combined with the DArT-Seq data of the population,five leaf rust resistance QTLs were mapped in this population.Lr13,from Apav,was detected in two environments and explained 51.60% and 25.08% of the phenotypic variance,respectively.Lr67 from KU3067 was detected in two environments,explaining 4.98%—7.30% of the phenotypic variance,respectively.QLr.hebau-2AS,from Apav,explained 6.68% of the phenotypic variance.QLr.hebau-5DL.1 from Apav,and QLr.hebau-5DL.2 from KU3067 explained 23.74% and 8.41% of the phenotypic variance,respectively,the two gene might be novel.This study clarified the resistance performance characteristics of the KU3067/Apav population in the Baoding environment,mapped multiple genetic loci including Lr13,Lr67,and three potentially novel leaf rust resistance QTLs,and developed closely linked molecular markers.This provides valuable genetic resources and markers for wheat leaf rust resistance breeding in China.

Key words: Wheat leaf rust, QTL mapping, Molecular marker, Genetic analysis

CLC Number:

XU Hao, DONG Haijiao, WANG Weixing, GAO Pu, WANG Xinhai, WANG Jinshuo, WANG Xinrui, LI Zaifeng, ZHANG Peipei. Gene Mapping for Leaf Rust Resistance in the Segregating Population of Common Wheat KU3067 and Apav[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(2): 179-186. doi: 10.7668/hbnxb.20196231.

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References 37

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参数Parameter	THJT	PHKT	THHT
抗病家系数量No.of homozygous resistant lines	102	99	100
感病家系数量No.of homozygous susceptible lines	39	39	39
分离家系数量No.of heterozygous lines	7	7	7
未出苗家系数量No.of missing lines	0	3	2
卡方检测P值P value of χ² analysis	0.33	0.27	0.29

参数Parameter	THJT	PHKT	THHT
抗病家系数量No.of homozygous resistant lines	102	99	100
感病家系数量No.of homozygous susceptible lines	39	39	39
分离家系数量No.of heterozygous lines	7	7	7
未出苗家系数量No.of missing lines	0	3	2
卡方检测P值P value of χ² analysis	0.33	0.27	0.29

数量性状位点 QTL	环境 Environment	位置/cM Position	标记区间 Marker interval	物理位置/Mb Physical position	对数优势值 LOD	贡献率/% PVE	加性效应/% ADD	供给亲本 Contributing parent
QLr.hebau-2AS	2022BDLR	30.00	1091536\|F\|-0-43:C>T~ 100056096\|F\|-0-11:C>G	24.6~18.8	6.58	6.68	10.31	Apav
Lr13	2021BDLR	41.00	3533726\|F\|-0-24:G>T~ 1002952\|F\|-0-37:T>C	116.0~118.0	25.19	51.60	21.99	Apav
	2022BDLR	46.00	6036484\|F\|-0-18:G>A~ 544256\|F\|-0-47:T>A	139.0~161.7	19.97	25.08	19.76
Lr67	2021BDLR	12.19	1070439~2261300	234.4~434.2	4.96	7.30	-9.07	KU3067
	2022BDLR	13.19	2261300~4003136	434.2~435.0	5.00	4.98	-9.99
QLr.hebau-5DL.1	2022BDLR	72.00	986261~3961411	437.7~549.3	15.87	23.74	18.27	Apav
QLr.hebau-5DL.2	2022BDLR	199.00	1155409\|F\|-0-51:G>A~ 1048778\|F\|-0-62:C>T	443.8~461.6	7.38	8.41	-11.65	KU3067

数量性状位点 QTL	环境 Environment	位置/cM Position	标记区间 Marker interval	物理位置/Mb Physical position	对数优势值 LOD	贡献率/% PVE	加性效应/% ADD	供给亲本 Contributing parent
QLr.hebau-2AS	2022BDLR	30.00	1091536\|F\|-0-43:C>T~ 100056096\|F\|-0-11:C>G	24.6~18.8	6.58	6.68	10.31	Apav
Lr13	2021BDLR	41.00	3533726\|F\|-0-24:G>T~ 1002952\|F\|-0-37:T>C	116.0~118.0	25.19	51.60	21.99	Apav
	2022BDLR	46.00	6036484\|F\|-0-18:G>A~ 544256\|F\|-0-47:T>A	139.0~161.7	19.97	25.08	19.76
Lr67	2021BDLR	12.19	1070439~2261300	234.4~434.2	4.96	7.30	-9.07	KU3067
	2022BDLR	13.19	2261300~4003136	434.2~435.0	5.00	4.98	-9.99
QLr.hebau-5DL.1	2022BDLR	72.00	986261~3961411	437.7~549.3	15.87	23.74	18.27	Apav
QLr.hebau-5DL.2	2022BDLR	199.00	1155409\|F\|-0-51:G>A~ 1048778\|F\|-0-62:C>T	443.8~461.6	7.38	8.41	-11.65	KU3067

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