Mapping of Quantitative Trait Locus and Developing of Closely Linked Markers for Tillering in Foxtail Millet

doi:10.7668/hbnxb.2018.06.005

Abstract

Abstract: Tillering is an important trait related to yield in many crops,and is the same for yield improvement of foxtail millet. However, the genetic mechanism of tillering in foxtail millet (Setaria italica) remains largely unknown. To accelerate mapping of relevant QTLs or genes, a restriction site-associated DNA sequencing (RAD-seq) approach, MSTmap and WinQTLCart 2.5 were employed to identify QTLs responsible for tillering in two F₂ populations called Cross AJ (543 F₂ individuals) and Cross HC (131 F₂ individuals). A total of six QTLs including qAJTN1, qAJTN5, qAJTN7-1,qAJTN7-2, qAJTN7-3 and qAJTN9, were identified in Cross AJ, explaining 0.7%-9.8% of the phenotypic variance, and two QTLs including qHCTN5 and qHCTN7 were identified in Cross HC, explaining 1.4%-8.3% of the phenotypic variance. Of which qAJTN1, qAJTN5, qAJTN7-2, qAJTN9 and qHCTN5 were newly identified QTLs, and qAJTN7-1, qAJTN7-3 and qHCTN7 were consistent with previous reports. Meanwhile, we screened the insertions and deletions in the QTL regions compared with the reference genome, insertion-deletion (InDel) markers linked with tillering were developed. These results of this study will facilitate the revelation of the genetic mechanism of tillering and molecular marker-assisted breeding of foxtail millet.

Key words: Foxtail millet, Tillering, QTL mapping, Linked marker

CLC Number:

S515

DU Xiaofen, WANG Jun, LI Yunfei, WANG Zhilan, YUAN Guobao, DU Guohua, HAN Fang, PENG Jianxiang, ZHANG Wenna, CAI Wei, YUAN Feng, CUI Juduo, GUO Erhu, ZOU Hongfeng, ZHANG Linyi, PENG Shuzhong. Mapping of Quantitative Trait Locus and Developing of Closely Linked Markers for Tillering in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 33-41. doi: 10.7668/hbnxb.2018.06.005.

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