Abstract: The yield and quality of wheat,a kind of important food crops,are often influenced under the condition of biotic or abiotic stresses.Genetic engineering technology is an effective method for wheat quality improvement,however,the transformation rate of wheat is still relatively low by far.In this experiment, E.coli genomic DNA was as template to clone otsA and otsB genes by the mean of PCR method.Then the target genes were connected with p2300-GFP,which would lead to the construction of p2300-otsAB vector.On the basis of establishing a perfect regeneration system,the common wheat was as the material.The p2300-otsAB expression vector was introduced into the cells of mature embryo and callus of wheat by Agrobacterium-mediated method.And then the new variety of salt-tolerant transgenic wheat was obtained.The genetic transformation results showed that when the concentration of Agrobacterium was OD₆₀₀=0.5,the time of infection of mature embryo or callus was 30 min,and the co-culture time was 3 d,the transformation efficiency of wheat was the highest.The experimental results can provide the reference to improve the stress resistance of plants through genetic engineering in the future.

Key words: Wheat, otsA gene, otsB gene, Mature embryo, Callus, Agrobacterium

CLC Number: 

• S432.4

• Q78