Cloning and Expression of DnaJ Homolog Gene from Eruca sativa

doi:10.7668/hbnxb.2015.06.009

Abstract

Abstract: To study the self-compatibility of Eruca sativa,a cDNA segments were isolated from stigma of pre-bloom of SC using differential display reverse transcriptase polymerase chain reaction (DDRT-PCR).The sequence prediction and bioinformatics analysis indicated that this cDNA segments was highly homolog with DnaJ protein homolog gene AtJ3 ,so named esAtJ3 gene temporary.The full-length of cDNA was 297 bp,which contained 98 amino acids and a DnaJ-C conserved domain belonged to the plant DnaJ superfamily.The protein encoded by esAtJ3 gene had no signal peptide and was a hydrophilic protein,it was mainly consisted of random coils and α-helices,the expression analysis of esAtJ3 gene using Real time RT-PCR showed that esAtJ3 gene expression levels was highest in stigma of pre-bloom of SC,which might be relate to the self-compatibility of Eruca sativa.

Key words: Eruca sativa, DnaJ homolog gene, Differential gene, Expression analysis

CLC Number:

FANG Yan, YANG Gang, SUN Wan, ZENG Xiu-cun, WU Jun, LIU Zi-gang, YANG Ning-ning. Cloning and Expression of DnaJ Homolog Gene from Eruca sativa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 61-65. doi: 10.7668/hbnxb.2015.06.009.

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