Analysis of High Yielding and Nitrogen Use Efficiency Mechanism of Wheat Kn9204 Using Gene Microarray

doi:10.7668/hbnxb.2014.04.037

Abstract

Abstract: Variations in gene expression profiles in high yielding and nitrogen use efficiency wheat variety Kn 9204 and the control variety J411 in different developmental stages and different tissues were analyzed using Affymetrix wheat microarray.The results indicated that 2 955 differentially expressed genes were detected between the two varieties in root at booting stage,including 1 201 and 1 754 of genes were up-and down-regulated comparing Kn9204 to J411,respectively.2 738 differentially expressed genes were detected between the two varieties in flag leaf at booting stage,including 1 323 and 1 415 of genes were up-and down-regulated comparing Kn9204 to J411,respectively.Besides 2 294 differentially expressed genes were detected between the two varieties in flag leaf 10 days after anthesis,including 839 and 1 455 of genes were up-and down-regulated comparing Kn9204 to J411,respectively.There were 220 and 303 of genes up-and down-regulated comparing Kn9204 to J411 in all the three groups,including both root and flag leaf at booting stage,and flag leaf 10 days after anthesis,respectively.Semi quantitative RT-PCR was further used to confirm the results of microarray,and all the four genes gained the same results by the two methods,indicating that the microarray results were biologically reproducible.To further characterize these differentially expressed genes,we used Gene Ontology(GO) for their annotation.The results showed that the most significant GO classification(According to the biological process classification) of differentially expressed genes between Kn9204 and J411 in all the three groups(Root at booting stage,flag leaf at booting stage and flag leaf 10 days after anthesis) was cellular nitrogen compound metabolic process,indicating that the difference of cellular nitrogen compound metabolic process between Kn9204 and J411 was probably to be the molecular mechanism leading to yielding and nitrogen use efficiency difference between the two varieties.

Key words: Wheat, Microarray, Differentially expressed genes, Semi-quantitative RT-PCR

CLC Number:

S512
S143.2

SUN Li-jing, ZHANG Wei, JI Jun, LI Hui, LI Jun-ming. Analysis of High Yielding and Nitrogen Use Efficiency Mechanism of Wheat Kn9204 Using Gene Microarray[J]. journal1, 2014, 29(4): 213-220. doi: 10.7668/hbnxb.2014.04.037.

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