Abstract: The study on the pollen germination and pollen tube growth of castor was performed after artificial pollination via fluorescence microscopy observation in order to verify that the best time to perform genetic transfor -mation of castor by pollen-tube pathway method is 6-24 h after pollination. Plant expression vector pPZP 221 SGN contained gene GUS were transformed into castor varieties tongbi 5 through pollen-tube pathway. 85 strains of T₀ gen-eration were tested by PCR method and the positive strains is 10,positive rate is 11.8%.The results show it is pos-sible to perform transgenic by pollen-tube pathway. This provides some references for the application of transforma-tion technology by pollen-tube pathway in castor molecular breeding.

Key words: Castor, Pollen tube pathway, pPZP221SGN

CLC Number: 

• S565.6