Isolation， Identification and Promoting Effect of Phosphate-solubilizing Bacteria in Rhizosphere of Zhanhua Winter Jujube

doi:10.3969/j.issn.1000-7091.2013.03.037

Abstract

Abstract: Phosphate-solubilizing bacterium(PSB) was isolated， screened and identified from the rhizospheric soil of Zhanhua winter jujube( Zizyphus jujuba Mill．) ，and phosphate-dissolving characteristics of PSB were determined．By using the way of clear zone，phosphate-solubilizing bacteria from the rhizospheric soil were preliminarily isolated，and the high effective PSB was further screened by using molybdenum-antimony anti-spectrophotometric method． The PSB was identified by the 16S rDNA sequence analysis combined with morphological characteristics．We carried out the salt-tolerant test，phosphate-solubilizing characteristics of PSB and optimization of fermentation medium in liquid tricalcium phosphate medium． The phosphate-dissolving characteristics of PSB in the soil were carried out under the greenhouse． The results indicated that one strain of high effective PSB was screened and identified as Rhizobium sp． The strain had significant effects on improving the solubilizing phosphate content in the liquid medium and the soil． This study provided excellent strain resource for improving phosphorus supply of Zhanhua winter jujube and promoting its growth．

Key words: Key words: Zhanhua winter jujube, Rhizospheric soil, Phosphate-solubilizing bacteria, Isolation and identification

CLC Number:

S432.4

Liu Xuehong, Zhang Liyan, Fan Yanhui, Zhang Lan, HAO Yali, Liu Nannan. Isolation， Identification and Promoting Effect of Phosphate-solubilizing Bacteria in Rhizosphere of Zhanhua Winter Jujube[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 204-209. doi: 10.3969/j.issn.1000-7091.2013.03.037.

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http://www.hbnxb.net/EN/Y2013/V28/I3/204

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