Associations of PCR-RFLP in Pit-1/Hinf Ⅰ Gene with Growth Traits in Hybrid Cattle of Luxi Yellow Cattle and Limousin

doi:10.3969/j.issn.1000-7091.2012.05.017

Abstract

Abstract: Improving the grouth level and meat production of Luxi Yellow Cattle,this paper use PCR-RFLP technology to analysis the correlation of Pit-1 gene polymorphism and growth traits of 111 hybrid cattle of Luxi Yellow Cattle and Limousin.The results show that the Pit-1-HinfⅠ loci of 451 bp′s PCR products is polymorphism after enzymes Hinf Ⅰ enzyme,the allele frequency of A and B were 0.32 and 0.68,it is not fit for Hardy-Weinberg balance.The rump length of AA genotype is higher than AB and BB genotype within population(P0.01),it can be used one of candidate genes;The hip width of AB genotype is higher than AA and BB genotype(P0.05) within the group;But there are no differences among 3 genotypes in the head length,forehead width,chest circumference,chest deep and wide,body length,rump length,waist angular wide,shank circumference,body height,the cross department high,the hip higher(P0.05)

Key words: Pit-1, PCR-RFLP, Growth traits, Hybrid cattle of Lilu

CLC Number:

S823

LIU Gui-fen, WAN Fa-chun, LIU Gui-ping, LIU Xiao-mu, LU Mao-yang, TAN Xiu-wen, CHENG Hai-jian, SONG En-liang. Associations of PCR-RFLP in Pit-1/Hinf Ⅰ Gene with Growth Traits in Hybrid Cattle of Luxi Yellow Cattle and Limousin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 91-95. doi: 10.3969/j.issn.1000-7091.2012.05.017.

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