Isolation and Expression Analysis of a GmXIP in Soybean

doi:10.3969/j.issn.1000-7091.2012.04.003

Abstract

Abstract: XIP（ X Intrinsic Protein） subfamily protein, as a member of AQP （Aquaporin）, was recently identified in the genomes and/or expressed sequence tag （EST） libraries of different plants. In this study,based on the genome-wide aquaporin genes sequences obtainmg by perl programs,a GmXIP was identified from soybean genome, including an intron 385 bp in length and spliced via AG/AG pattern different from common plants GT/AG format; compared with from other plants XIP protein amino acids, GmXIP also contains conserved six transmembrane domains,however,the two conserved ＂NPA＂ motifs of MIP （Major Instrinsic Protein） character changed into ＂ NPI＂ and ＂ SPA＂,implying that solute transport of GmXIP carried maybe different from that of other plants; semi-QRT- PCR result indicated that the expression of GmXIP mainly focused on stem and leaf organs,but not flower and pod tissues; additionally, with the growth and development of plant, the expression in stem gradually increases and that in leaf keep higher expression, this demonstrated that this gene probably played important roles in the transport and supplying of water and nutrition;the expression get the highest at 8 h under salt treatment, and slowly decreased af- ter this time point;overexpression GmXIP transgenic composite plants appear more susceptibility compared with control under drought and salt treatments after 3 days,resulting from the speed of water loss in overexpression GmXIP plants got fast and led to plant death. This study will provide theatrical support and technology guide for elucidating the structure and function of soybean XIP.

Key words: XIP Intron splice, Gene expression, Transgenic composite plants

CLC Number:

HANG Da-yong, YI Jin-xin, HU Guo-min, XU Ling, YUAN Ling-ling, XU Zhao-long, HE Xiao-lan, HUANG Yi-hong, LIU Xiao-qing, MA Hong-xaang. Isolation and Expression Analysis of a GmXIP in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 12-17. doi: 10.3969/j.issn.1000-7091.2012.04.003.

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References

[1] Agre P, Kozono D. Aquaporin water channels:molecular mechanisms for human diseases [J]. FEBS Lett, 2003, 555:72 -78.

[2] Johanson U, Karlsson M, Johansson I,et al. The complete set of genes encoding major intrinsic proteins in Arabidop- sis provides a framework for a new nomenclature for major intrinsic proteins in plants [J]. Plant Physiol, 2001,126 : 1358 - 1369.

[3] Gupta A B, Sankararamakrishnan R. Genome-wide analy- sis of major intrinsic proteins in the tree plant Populus tri- chocarpa:characterization of XIP subfamily of aquaporins from evolutionary perspective [J]. BMC Plant Biol,2009, 9:134.

[4] Park W, Scheffler B E, Bauer P J,et al. Identification of the family of aquaporin genes and their expression in up- land cotton (Gossypium hirsutum L). BMC Plant Biol,2010,10:142.

[5] Chaumont F, Barrieu F, Wojcik E, et al. Aquaporins con- stitute a large and highly divergent protein family in maize [J]. Plant Physio1,2001,125 : 1206 - 1215.

[6] Zelazny E, Miecielica U, Borst J W, et al. An N-terminal diacidie motif is required for the trafficking of maize aqua- porins ZmPIP2;4 and ZmPIP2;5 to the plasma membrane [J]. Plant J,2009,57(2) :346 -355.

[7] Li D D,Wu Y J,Ruan X M,et al. Expression ofthree cot- ton genes encoding the PIP proteins are regulated in root development and in response to stresses[J]. Plant Cell Rep,2009,28(2):291 -300.
[8] Matsumoto T,Lian H L,Su W A,et al. Role of the aqua- porin PIP1 subfamily in the chilling tolerance of rice[J]. Plant Cell Physiol,2009,50( 2 ) :216 - 229.

[9] Gattolin S, Sorieul M, Hunter P R,et al. In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots [J]. BMC Plant Biol,2009,9:13.

[10] Liu Q, Wang H, Zhang Z, et al. Divergence in function and expression of the NOD26-like intrinsic proteins in plants[J]. BMC Genomies,2009,10:313.
[11] Fotiadis D, Jen P, Mini T, et al. Structural characteriza- tion of two aquaporins isolated from native spinach leaf plasma membranes[J]. J Biol Chem, 2001, 276 : 1707 - 1714.

[12] Danielson J A, Johanson U. Unexpected complexity of the aquaporin gene family in the moss Physcomitrella patens [J]. BMC Plant Biol,2008,8:45.

[13] Javot H, Maurel C. The role of aquaporins in root water uptake. Ann Bot. 2002,90 : 301 - 313.

[14] Heinen R B, Ye Q, Chaumont F. Role of aquaporins in leaf physiology[J]. J Exp Bot, 2009,60 ( 11 ) : 2971 - 2985.

[15] Forrest K L,Bhave M. Major intrinsic proteins (MIPs) in plants:a complex gene family with major impacts on plant phenotype [J]. Functional & Integrative Genomics, 2006,7 ( 4 ) : 263 - 289.

[16] Kaldenhoff R, Fischer M. Aquaporins in plants [J]. Acta Physiology,2007,187 : 169 - 176.

[17] Aharon R, Shahak Y, Wininger S, et al. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress [J]. Plant Cell, 2000,15:439 - 447.

[18] Sakr S, Alves G, Morillon RL, et al. Plasma membrane aquaporins are involved in winter embolism recovery in walnut tree[J]. Plant Physiology,2003,133:630 - 641.

[19] Roussel H, Bruns S, Gianinazzi-Pearson V, et al. Induc- tion of a membrane intrinsic protein-encoding mRNA in arbuscular mycorrhiza andelicitor-stimulated cell suspen- sion cultures of parsley [J]. Plant Sci, 1997,126 : 203 - 210.

[20] Krajinski F, Biela A, Schubert D, et al. Arbuscular my- corrhiza development regulates the mRNA abundance of Mtaqpl encoding a mercury-insensitive aquaporin of Medicago truncatula[J]. Planta,2000,211:85 - 90.

[21] Chrispeels M J, Crawford N M,Schroeder J I,et al. Pro- teins for transport of water and mineral nutrients across the membranes of plant cells [J]. Plant Cell, 1999,11 : 661 - 665.

[22] Azaizeh H,Steudle E. Effects of salinity on water trans- port of excised maize roots[J]. Plant Physiol, 1991,97 : 1136 - 1145.

[23] Bienert G P, Bienert M D, Jahn T P, et al. Solanaceae XIPs are plasma membrane aquaporins that facilitate the transport of many uncharged substrates[J]. The Plant J, 2011,66:306 -317.

[24] Tornroth-HorseLeld S, Wang Y, Hedfalk K, et al. Struc- tural mechanism of plant aquaporin gating. Nature,2006, 439:688 - 694.

[25] Sade N, Vinocur B J, Diber A S,et al. Improving plant stress tolerance and yield production: is the tonoplast aquaporin SITIP2;2 a key to isohydric to anisohydric conversion[J]. New Phytol,2009,181:651 - 661.

[26] Shelden M C, Howitt S M, Kaiser B N,et al. Identifica- tion and functional characterisation of aquaporins in the grapevine, Vitis vinifera[J]. Funct Plant Biol, 2009,36 : 1065 - 1078.

[27] Yamaguchi-Shinozaki K, Koizumi M, Urao S, et al. Mo- lecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana:sequence analysis of one cDNA clone that en- codes a putative transmembrane channel protein [J]. Plant Cell Physiol, 1992,33 : 217 - 224.

[28] Yamada S, Komory T, Myers P M, et al. Expression of plasma membrane water channel genes under water stress in Nicotiana excelsior [J]. Plant Cell Physiol, 1997,38 : 1226 - 1231.

[29] 《基于Perl脚本的大豆核苷酸序列高通量提取》[J].南京农业大学学报,2012(1).

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