Sequencing and Analysis of CDT Gene of Haemophilus parasuis Clinical Isolates

doi:10.7668/hbnxb.2011.06.015

Abstract

Abstract: Cytolethal distending toxin(CDT) is a virulence factor of gram negative bacteria． The aim of this study was to investigate the prevalence and phylogenetic relationships of CDT genes in different clinical isolates of Haemophilus parasuis． The presence of CDT gene was detected by PCR against three subunits gene of the toxin respectively． Of the 41 clinical isolates from pig farms of Zhejiang province，CDT genes of 21 isolates were sequenced． It shows that CDT gene of three subunits were presented in the genome of all 41 isolates used in this study． CdtA is the most conserved protein with more than 97． 8% amino acid sequence identity compared to cdtB and cdtC(both≥ 92． 1%). The catalytic residues in cdtB corresponding to bovine DNase I are highly conserved among all the isolates， which may indicate a key role of cdtB in the activity to induce mammalian cell apoptosis． This artical provide new information which will facilitate study of the biological activity of Haemophilus parasuisCDT．

Key words: Haemophilus parasuis, CDT, Identity

CLC Number:

LI Jun-xing, WANG Yi-cheng, YUAN Xiu-fang, XU Li-hua, CAO Hui-min, SHEN Shi-chuan, ZHANG Lu-bin. Sequencing and Analysis of CDT Gene of Haemophilus parasuis Clinical Isolates[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 79-84. doi: 10.7668/hbnxb.2011.06.015.

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http://www.hbnxb.net/EN/Y2011/V26/I6/79

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