Cloning and Analysis of Upland Cotton(Gossypium hirsutum) Dirigent-like Gene(GhDIR)

doi:10.7668/hbnxb.2011.05.007

Abstract

Abstract: Dirigent-like gene encodes a protein invloved in the formation of lignin,and in defence to pathogen disease.Using an EST of a dirigent-like gene as the query probe to blast cotton EST database,a full-length 768 bp cDNA sequence of the dirigent-like gene was obtained and cloned by PCR,was named GhDIR.Sequence analysis indicated that it contained a complete open reading fragment(ORF),from 70 to 594 bp,encoding 175 amino acids,and has no intron.Sequence comparison and phylogenetic analysis showed the GhDIR cDNA was highly identical with its ortholog from Sea Island cotton,and the next most similar orthologs coming from other dicotyledons,but sharing low homology with monocotyledons.Quantitative PCR analysis showed that GhDIR was activated in roots of Upland cotton inoculated with Verticillium dahliae.

Key words: Gossypium hirsutum, In silica cloning, DIR-like gene, Expression analysis, Vertivillium dahlige

CLC Number:

S562

ZHAO Fu-an, FANG Wei-ping, YANG Xiao-jie, XIE De-yi, LI Wu, TANG Zhong-jie. Cloning and Analysis of Upland Cotton(Gossypium hirsutum) Dirigent-like Gene(GhDIR)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 29-33. doi: 10.7668/hbnxb.2011.05.007.

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