Research on CaM and Its Isform Genes Involved in the Resistance Response of Wheat to Puccinia triticina

doi:10.7668/hbnxb.2010.04.037

Abstract

Abstract: CaM is involved in plant resistance response. To study the differential expression of CaM and four isforms gene in wheat TcLr15 after inoculation with virulent race and avirulent race，r elatively quantitative analysis was performed by real-time PCR. The level of CaM and four CaM isforms gene expression in wheat TcLr15 was tested by real-time Fluorescent Quantitative PCR (real-time PCR) after inoculation with virulent race and avirulent race respectively. No significant difference in the gene expression of CaM and its four isoforms was observed between the detached wheat leaves inoculated with virulent and without until 12 h after inoculation. The gene expression of CaM SF-1 in wheat at 48 h，72h and 96 h after inoculation with avirulent race 05-5-127 ③ were increased by 24.6%，26.6% and 38. 8% compared with that of inoculation virulent race 05-22-64 ①. The gene expression of,CaM SF-4 at 24 h， 48 h after inoculation with avirulent race 05-5-127 ③ were increased by 26. 8% and 28. 0% if compared to that of inoculation with viruent race 05-22-64 ①. The gene expression of CaM SF-2 in the incompatible interaction was lower than that of compatible interaction control from 24 h to 96 h after inoculation. No obvious difference in gene expression level of CaM SF-3 between compatible and incompatible interaction was found. The gene expression of CaM in wheat after inoculation with avirulent race 05-5-127 ③ at the 24h and 48h were increased by 18.1% and 47.9% if compared to that of inoculation with virulent race 05-22-64 ①，however，the gene expressive quantity of CaM in wheat at 72 h and 96 h after inoculation with avirulent race 05-5-127 ③ was lower than that of inoculation with virulent race 05-22- 64 ①. Taken together，these data implied that CaM might be involved in the regulation on wheat resistance against its leaf rust disease. More over，there exist a CaM isoform specificity for the involving in wheat defense against the infection of Puccinia triticina. The CaM SF-1 and CaM SF-4 genes may be related to wheat against the infection of puccinia triticina. The CaM SF-2 gene may be related to wheat susceptible to the infection of Puccinia triticina. The CaM SF-3 gene may be not related to wheat against the infection of puccinia triticina.

Key words: Wheat, Puccinia triticina, CaM, Isforms, Real-time PCR

CLC Number:

S432.2+3

HUO Jian-fei, SONG Shui-shan, LI Xing, YANG Wen-xiang, LIU Da-qun. Research on CaM and Its Isform Genes Involved in the Resistance Response of Wheat to Puccinia triticina[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 175-179. doi: 10.7668/hbnxb.2010.04.037.

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