Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage

doi:10.7668/hbnxb.2010.03.019

Abstract

Abstract: The objective of the present study is to construct a genetic linkage map associated with internationally agreed reference linkage map in Chinese cabbage ( Brassica rapa L.ssp.pekinensis).183 DH lines derived from a cross of two DH lines,Y177-12 and Y195-93,were used for map construction.Six kinds of marker systems including SRAP, SSR,AFLP, STS,ESTP and CAPS loci were used for polymorphic marker screening.The assignment of linkage groups to their chromosomes was determined by using markers which have been mapped in the reference map.A genetic linkage map was constructed for Chinese cabbage, covering 1 036 cM,with an average mapping interval of 4.4 cM.233 loci including 145 SRAP, 62 SSR, 12 AFLP,6 STS,4 ESTP,1 CAPS and 3 morphological markers were integrated into 10 linkage groups using JoinMap 3.0.38 SSRs,4 ESTPs and 2 STSs provided anchors to previously published linkage map for B.rapa and B.napus and the consensus nomenclature of A1-A10 was adopted in this study.A genetic linkage map of Chinese cabbage associated with chromosomes was constructed,which enabled the alignments and integration between sets of maps and populations and would be useful in gene localization, comparative genomes research and QTL mapping of important agronomic traits for Chinese cabbage．

Key words: Chinese cabbage, Molecular marker, Genetic linkage map, Chromosome assignment

CLC Number:

S634.03

YUAN Yu-xiang, ZHANG Xiao-wei, SUN Ri-fei, WANG Xiao-wu, ZHANG Hui, JIANG Wu-sheng, YAO Qiu-ju, GENG Jian-feng. Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 80-86. doi: 10.7668/hbnxb.2010.03.019.

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