Optimizing of AFLP Analysis System for Sweet Cherry and Screening Polymorphism Primers

doi:10.7668/hbnxb.2010.02.024

Abstract

Abstract: The variety Heizhenzhu and Shamidou were used as materials for establishing DNA-AFLP system.Several key factors affecting DNA-AFLP analysis were detected such as DNA extraction,enzymes restriction,adaptor-ligation,pre-amplification and selective amplification.The results indicated that SDS method could be used for extracting ideal DNA from leaf of sweet cherry;500 ng DNA was digested completely by EcoR I and Mse I for 4 h.The reducible results was obtained when the ligation products were diluted to 5 times for pre-amplification template and pre-amplification products were diluted to 20 times for selective amplification.This study established a foundation for studying the cherry genetic diversity and QTL for cherry agricultural character.

Key words: Sweet cherry, AFLP

CLC Number:

S662.5

ZHANG Chao-jie, XU Wei-hua, LIU Wan-hao, TANG Mei-ling. Optimizing of AFLP Analysis System for Sweet Cherry and Screening Polymorphism Primers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 125-128. doi: 10.7668/hbnxb.2010.02.024.

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