Abstract: With a F₂and BC1population derived from F₁of B180H(yellow cotyledon female)and Q12(green?cotyledon male)was used as materials to study the inheritance of yellow cotyledon color and molecular marker associated to the cotyledon yellow trait. The results indicated that the yellow cotyledon trait was controlled by a pair of?recessive gene，and the green cotyledon trait was in the mode of incomplete dominant inheritance. The polymorphism?between yellow and green cotyledon parents of cucumber were studied using BSA method and AFLP technology.?1 024 AFLP markers were screened，and a co-dominant marker E-AG/M-AAG was obtained. Sequencing of the?polymorphic fragment indicated that their lengths were 258 bp and 257 bp，respectively，and they were orthologous?sequences with an insertion /deletion or mutant of only one nucleotide. The yellow cotyledon F₂plant possessed the?258 bp fragments，and the green cotyledon F₂plants possessed the 257 bp fragments or possessed both two fragments. The marker was closely linked to the cucumber cotyledon yellow color trait-related gene，and the marker was?confirmed to be 96. 74% accurate by F₂individuals. The trait was a useful marker for hybridization and purity identification of F₁hybrids.

Key words: Cucumber, Cotyledon color, Inheritance, Molecular marker, AFLP

CLC Number: 

• S642.2