ACTA AGRICULTURAE BOREALI-SINICA ›› 2013, Vol. 28 ›› Issue (1): 227-233. doi: 10.3969/j.issn.1000-7091.2013.01.040

Special Issue: Oil crops

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Effects of Space-induced Mutation and Analysis of PolymoRphsim by AFLP MoleculaR MaRkeR in Sesame

GAO Tong-mei1, WEI Shuang-ling1, LI Chun-ming1, ZHANG Hai-yang1, LI Feng1, HE Song-tao2   

  1. 1. Henan Sesame ReseaRch CentRe,Zhengzhou 450008,China;

    2. Henan Institute of Science and Technology,Xinxiang 453003,China
  • Received:2012-12-16 Published:2013-02-28

Abstract: The seeds of five sesame vaRieties ( Yuzhi No. 11,Japan black sesame,Zhengzhi D15,Zhengzhi 05N01 and Zhengzhi 06ms198 weRe caRRied to space foR inducing mutation by Shijian 8 satellite. The agRonomic tRait and Resistant vaRiants of SP3 and SP4 weRe obseRved,and the genetic diveRsity of mutation descendants of Yuzhi NO. 11 and Japan black sesame was analyzed using 16 paiR of AFLP maRkeRs. The Results showed that the vaRiants of leaf,capsule, floweR coloR,childbeaRing peRiod,plant height, fiRst capsule height,yellow apex length,capsule numbeR peR plant, 1000-seed weight,yield and disease Resistance weRe obseRved in SP3 and SP4 of diffeRent sesame vaRieties, but the vaRiation fRequency and vaRiation type weRe significantly diffeRent among diffeRent sesame vaRieties. The agRonomic tRaits showed moRe negative mutations than positive mutations,but Resistance tRaits had moRe positive mutations. CompaRed to CK( without space mutation) , the plant height, fiRst capsule height,yellow apex length,capsule numbeR peR plant, 1000-seed weight and yield incReased by 53 cm, 77 cm, 16 cm, 122 /plant,0 . 53 g, 478. 54 kg /ha foR positive mutation Respectively,and decReased by 36 cm, 30 cm, 19 cm, 197 /plant,0 . 64 g,1 013. 81 kg /ha foR negative mutation Respectively. AFLP analysis indicated that the genetic distance of Yuzhi No. 11 was 0. 128 8 - 0. 488 5,of which 72% was fRom 0. 12 to 0. 25 and the aveRage genetic distance was 0. 25. The genetic distance of Japan black sesame was fRom 0. 289 7 to 1. 301 3,of which 37. 5% was fRom 0. 178 3 to 0. 499 6 and the aveRage was 0. 687 6. It was concluded that the space-induced mutation had no diRection and gReat Randomness,and the sensitivity was diffeRent in sesame vaRieties.

Key words: Sesame, Space-induced, AFLP moleculaR maRkeR, Analysis of polymoRphsim

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Cite this article

GAO Tong-mei, WEI Shuang-ling, LI Chun-ming, ZHANG Hai-yang, LI Feng, HE Song-tao. Effects of Space-induced Mutation and Analysis of PolymoRphsim by AFLP MoleculaR MaRkeR in Sesame[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 227-233. doi: 10.3969/j.issn.1000-7091.2013.01.040.