A Construction of AFLP Analysis System for Dwarf Cucurbita moschata Research

doi:10.7668/hbnxb.2011.02.027

Abstract

Abstract: Based on dwarf and vine inbred lines,key factors of AFLP analysis system(such as genomic DNA extraction,time requirement of digestion and ligation reactions,dilution of pre-amplification and silver staining method et a1)were studied.The result showed：the best quality genomic DNA were adding PVP and B-mercaptoethanol modified CTAB method；5.5 h can be meet digestion and ligation reactions；Diluted 5 times for products of digestion and ligation reactions and diluted 30 times for products of pre-amplification can be meet experimental conditions.96 primers screened,reproduce,clear polymorphic bands were obtained between dwarf and vine inbred lines.It laid good foundation to tropic pumpkin dwarf vine mapping.

Key words: Tropic pumpkin, Dwarf, AFLP analysis system

CLC Number:

S62.1

WU Jun-xin, CHANG Zhi-jian. A Construction of AFLP Analysis System for Dwarf Cucurbita moschata Research[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 124-127. doi: 10.7668/hbnxb.2011.02.027.

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