New Method to Identify 1BL/1RS Translocation in Common Wheat

doi:10.7668/hbnxb.2009.S1.009

Abstract

Abstract: To develop a new method for 1BL/1RS translocation identification with mixograph parameters,404 advanced lines(Experiment I)and 175main cultivars of Shandong Province and advanced lines(Experiment II)were used in this study.The genetic effect of 1BL/1RS translocation on mixograph parameters was investigated .1BL/1RS translocation lines showed significantly shorter mixing time,less bandwidth of peak and bandwidth after 1 min peak,and higher angle of descent and the bandwidth ratio(the ratio of bandwidth of peak/bandwidth after 1 min peak)in comparison with non -1BL/1RS translocation lines .It indicated that the 1BL/1RS translocation has deleterious effects on mixograph parameters . Mixograph of the 1BL/1RS translocation was characterized with the bandwidth sharply declining and narrowing after 1min peak,and increasing the bandwidth ratio ,whereas the bandwidth of non -1BL/1RS translocations declined gently after 1 min peak or had a longer mixing tolerance,and had a little variation about the bandwidth ratio .Furthermore,85 .2 %(Experiment I)and 96 .8 %(Experiment II)accuracies were achieved in grouping the 1BL/1RS translocation and non -1BL/1RS translocation on the basis of the band width ratio,i .e ., 1BL/1RS translocation line had a value more than or equal to 1 .6,and non -1BL/1RS translocation line had a value smaller than 1 .6 .Therefore ,mixogram and its parameters could be used as a new method to determine the presence of 1BL/1RS translocation quickly and efficiently.

Key words: Commonwheat(TriticumaestivumL.), 1BL/1RStranslocation, Mixogramcharacteristics

CLC Number:

S512.03

CHENGDun-gong, LIHao-sheng, XIAOYong-gui, SONGJian-min, LIUAi-feng, LIUJian-jun. New Method to Identify 1BL/1RS Translocation in Common Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.009.

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