Cloning and Expression Analysis of Flavonoid 3-O-gulcosyltransferase Gene from Potato

doi:10.7668/hbnxb.2009.04.003

Abstract

Abstract: The degenerate primers were designed based on the published information of sequence of Solanaceae plants. The cDNA clones encoding UDP2glucose : flavonoid 32O2glucosyltransferase ( 3GT) have been isolated from Solanum tuberosum cv. Chieftain with the degenerate primers by RT2PCR. The cDNA clone of 3GT encodes a predicted polypeptides of 448 amino acids,and the deduce protein showed 76 % highest identity with 3GT of Petunia hybrida and Solanum melongena,and the multiple alignment and phylogenetic analysis demonstrated that it belong to a new member of the corresponding 3GT2family enzymes. The mRNA expression analysis demonstrated that the accumulation of 3GT tran2 scripts in root,leaf and tuber was much more than that in stem and flower. The accumulation of 3GT transcripts in red2 pigmented callus was much more than that in non2pigmented callus of S. tuberosum cv. Chieftain. The expression of 3GT gene followed by anthocyanin accumulation.

Key words: Anthocyanins, cDNA cloning, Expression pattern, Solanum tuberosum, UDP2glucose :flavonoid 32O2glu2 cosyltransferase (3GT)

CLC Number:

Q785

LU Qi-neng, YANG Qing, SHEN Chun-xiu. Cloning and Expression Analysis of Flavonoid 3-O-gulcosyltransferase Gene from Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 11-16. doi: 10.7668/hbnxb.2009.04.003.

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